The Experiments of Twilight Sparkle (revised)

"Well, that's just great," Twilight spoke. "It's too late to amputate her leg to save her. The poison already spread. She'll be dead soon, if I don't inject her with the vaccine. I can't have her die."

Twilight took a hypodermic needle from one of the cases are gave the dying Pinkie a shot. "It should take effect soon."

Twilight poured a disinfectant liquid onto a gauze sponge and cleaned Pinkie's wound made from the hacksaw. After she sewed the wound closed with a suture and an absorbable thread, and rapped a bandaged around the wound, she took notice of the light radiating from Dash's element of loyalty. "Rainbow, your necklace is glowing. You're beginning to show your loyalty to your pink friend just by having a strong desire to free Pinkie. Well, let's see just how bright you can get that light to glow without actually fulfilling your loyalty by setting her free. But rather through your desire alone. On to lesson zero, ur, two."

The whole group saw Twilight put on a surgical mask, open a metal case, reach inside, and take out what looked like a surgical knife. Everyone's eyes grew wide with concern.

Pinkie was feeling the relieving effect of the vaccine "Twi. Please, please tell me that that knife is to cut a cake. And that that's a baker's apron. Please tell me that this is all a plank... A meany-mean prank."

"Oh, Pinkie." Twilight chuckled. After giving Pinkie a kiss on her forehead, she held the scalpel close up to Pinkie's face. "Here's for science."

"Heh heh. Careful with that knife." Pinkie said nervously.

Dash's heart beat significantly faster at the sight of a knife that close to her friend. "Twilight. I don't want to do this! Please let me go." Yelled Dash.

Twilight was vexed. "Why not? Your silly nightmare?"

Dash replied, "This is getting more and more like that nightmare. What exactly do you plan on doing with that knife anyway?"

Applejack joined in. "What's gotten into you?! You're worrying us Twi."

Twilight sighed in frustration. "It's for science, for the last time!"

Pinkie watched as the knife hovered down her chest and to her belly. "Shouldn't you be baking that cake now?" Pinkie said, trying to convince herself that this was all some sort of sick joke.

The knife caressed the skin of Pinkie's chest. Suddenly, Twilight pushed harder on the knife, and the extremely sharp tool cut through the skin and a little of the muscle of the pink pony's chest. "AHUG!" Pinkie yelled. Then Twilight slowly moved the blade straight down to the belly, making a deep horizontal gash through the skin and layers of muscle, causing Pinkie to scream in pain. It was not long before red liquid was seeping out of the horizontal gash.

There was a sudden adrenaline rush in everyone, from seeing what just happened. "TWILIGHT!?" Both Rarity and AJ hollered.

Rainbow Dash was now filled with panic. "GET US OUTTA HERE!" Dash screamed, while jerking franticly, trying to break free from the metal cuffs, but to no avail. The light from her necklace brightened.

The surgeon used a gauze sponge wet with a disinfectant to wipe the long cut to prevent infection. The disinfectant stunk Pinkie badly, "aaaAAAAAHHH! AAAAAHHHHH!" Not wanting Pinkie to lose too much blood, she used some clamps to clamp the blood vessels where the incisions were. Twilight used her hoof to lift one of the flaps of Pinkie's flesh and meat made by her huge wound on her underside. She stretched, compressed, and wiggled the mass of meat and skin as if she were studying it. Pinkie let out a whine of great discomfort with each burning stretch and twist.

Fluttershy shouted, "LEAVE PINKIE ALONE! You're hurting her... badly! Just listen to her cries of pain. Doesn't that say anything to you?"

"Oh, she's just being Pinkie Pie," was Twilight's response.

Fluttershy continued, "Why did you cut Pinkie's belly open? She's your friend!"

"Fine." Twi sighed. "If I must spell it out for you. She is my friend. That is why we're learning together. All of us. I opened Pink it increase Dash's desire to rescue her, thus showing more and more of her loyalty, which will manifest physical through the light glowing from her helmet. I might even remove Pinkie's organs after the lesson." Twilight gave a smile, eyes closed and head up high, showing how proud she was that she would be able to do this like a real scientist. The five ponies were utterly shocked at what they just heard.

"Once we're done learning about Pink and Dashie, then we shall move on to AJ, Shy, and Rar. When we are finished, more will be known about the elements of harmony and the magic of friendship than ever before!"

All of them were breathing heavily and fast. Their heart's were pounding. Panic was in the air. "I'll be back in a sec." said Twilight. They all shouted and yelled pleas for mercy just as Twilight left the room.

Upon returning, she dragged a huge multi-layered cart with an array of glass containers filled with a clear liquid. The five ponies were petrified at the freaky and unnerving sight before them. Some jars had active pony organs already inside of them. The hearts were still beating. The lungs were still expanding and compressing. Stomachs were still contracting their muscles. As if the liquid in the containers was some sort of bioelectricity inducing substance. The glass bottles had labels on them. They labeled the type of organs that were in them, and ponies they belonged to. The horrified mares read the labels. Some empty ones (but still with liquid) read Pinkie Pie. Some containing organs read Blue October, Pokey Pierce, and... Apple bloom!

Applejack's world was further crashing down, "YOU KILLED HER! YOU KILLED MY KID SISTER! SHE'S WAS JUST A LITTLE HELPLESS FILLY!" Tears were flowing down her cheeks.

The killer explained, "Well, yeah. I needed practice before I got to Pinkie. Science is full of trial and error." AJ's throat moistened and she found it hard to swallow. All sorts of intense emotions ran through her. Shock. Fear. Despair. RAGE!

"You know," Twilight went on, "it's interesting, this taboo of society. Ponies are always more sensitive about young foals being hurt and killed than adults. Why do you think that is?"

Twilight Grabbed a metal, several silver pins from an open metal case. Pinkie didn't want to know what the unicorn was going to use them for. "Twilight, no! NO! PLEASE NO!"

After resting the pin's tip on the inside of one of the flaps of meat, Twilight leaned over and kissed Pinkie on the forehead. Then she slammed her hoof to the top of the pin. The pointed object pinned down one of Pinkie Pie's flaps of skin to the table. The pin rested in a hole pre-embedded in the table.

"AAAAAAAAAAAAAAAAA," Pinkie felt a high concentration of pain where the flap was pinned down. Twi took two more of the sharp pins and forcefully pushed them into flap to hold it down more securely. Pinkie let out a high pitched shriek with each sudden, blood squirting stab into the meat and flesh. Streams of tears were flowing down the party mare's face. Taking three more nails, she did the same to the flap on the pink pony's other side. This left Pinkie's ribcage and bottom organs exposed. Rarity felt nauseous. But Twilight wasn't finished.

She took out a small circular saw out of a case. It was a bone cutter. She turned it on and pushed the spinning saw into the bottom of Pinkie's blood covered sternum. The loud cutting noise could be heard throughout the dungeon. Sawing straight up through the hard chest bone, countless red drops sprayed onto Pinkie's tear soaked face and on Twilight's shy blue surgeon's apron and mask.

The crying, begging, and pleading for the purple unicorn to stop, from the four other ponies, were relentless. Applejack pleaded, "ANYTHING! ANYTHING AT ALL! NAME IT. WE'LL DO IT. PLEASE JUST STOP THIS!"

Dash shouted, "WE LOVE YOU PINKIE! WE'LL GET YOU OUT OF THIS! WE SWEAR!" The light glowing brighter still.

Rarity implored, "No, NO! NO MORE! NNNOOOO!"

Fluttershy was of course crying the hardest, "Pinkie Pie is your friend! How could you do this to poor Pinkie? Please PUT HER BACK TOGETHER!" But they didn't seem to phase Twilight at all, except for her horn. Which looked as though it sharpened.

With Pinkie's sternum split, the surgeon unicorn inserted a rib spreader into the crevice and turned the lever enough to push the two half sternums a bit apart, leaving Pinkie's beating heart in plain sight. But the ribs were not spread apart too much, as to allow her lungs to expand and compress. Pinkie was too frightened to look down. Twi locked the lever in place.

Next, she brought out what looked like a huge basting bulb and explained, "See this? It contains alcohol. I'm going to pour it all over your exposed internal organs to prevent infection. You don't expect me to experiment on infected organs, do you? This is going to sting... a lot. Brace yourself!"

"No... Twi... no...," Pinkie spoke weakly from some blood loss and the stress of the pain, her face wet with blood and tears. As if Twilight didn't even hear her, she squirted the infernal liquid all over poor Pinkie Pie's bare insides. Sssstiiiinnnngggg! The pain was so intense, it was like nothing she had ever suffered before. Deafening, blood curdling screams of sheer agony echoed off the cave walls. "THIS IS NOT RRRIIIGGHHT!" cried the suffering mare.

Twilight repeatedly rubbed her cheek against her sobbing victim's, and gave some kisses in between, "Shh, shh, sh, shhhh. It's okay. It's going to be alright."

The four other captives were in such trauma. They couldn't stand seeing their best friend in so much pain. Pinkie looked so weak and helpless. It was too much for them to handle. Fluttershy threw up and blacked out.

Twilight removed her mask. Pinkie weakly glanced at her captor. Putting her face right up to Pinkie's, Miss Sparkle's irises fluctuated between dark red and bright red as she spoke with a big, happy smile. "Guess what. I KILLED Applebloom! I killed her. And I'm going to KILL you too! And it makes me happy! Your cutie mark does not lie. Your parties do give ponies smiles. And what a party this is! Tell me, how does all this make you feel, emotionally? Does this change your naïve, happy-go-lucky view on this world we live in?" The unicorn waited for a response from the miserable pink pony.

After a few moments, the pitiful pony let out sobs of emotional trauma. She felt like nothing could stop this. Not even laughter.

When she finished, Twilight spoke with intrigue, "Interesting. The magical energy seems to be dwindling to nothing. You've finally learned that laughter will not always make the monsters disappear. I always knew that laughter was the least useful element. But it is the most fun! HA HA HA HA HA!"

Twilight addressed everyone, making sure they were all listening. "That's it. We all learned something new about our friend Pinkie Pie. And that is that, under such horrific circumstances like this, she's loosing her element. How sad, heehee. But we are not done. There is more to be learned about Pinkie, and at the same time, Dash's element of loyalty."

When she was finished, she put back on her surgical mask, readied the glass containers, then sterilized her hooves. The ponies made one last attempt to reason with the heartless horse. Applejack tried first, "Twi. Ya don't have to do this. It's not too late. Just stitch Pink back together."

The light from Dash's helmet increased a little more in intensity. "Yes, Twi. She's your friend. We know you really don't want to do this."

Rarity was next, "We don't know what has gotten into you, but please, let us help you. We can help. We love you dear."

Twilight was grateful for their offer, "Thank you all. You are my dear friends." There was a spark of hope in them. Twilight's eyes turned an even brighter red than before, "But there is nothing wrong with me." Everyone's heart sank. Then came the begging and sobbing again.

Twilight did not seem to care, as she opened the bottle and grabbed the party pony's stomach. Pinkie pie felt sick to the stomach, that is until Twilight gave it a good yank out of her, carrying with it part of the esophagus. "Uuugggk!" reacted Pinkie. Twilight immediately put the digestive organ into the preservative fluid filled jar and closed the lid.

Twilight made a discovery, "Well you had a big stomach. Which is to be expected, since you ate a lot of goods." Twilight went for her liver next. "This organ absorbs lipids from Pinkie pie's warm, sweet blood. Lipids serve as an energy reserve. And the liver is where they are stored." Twilight proceeded with the pancreas, gallbladder, spleen, and small intestine, giving brief facts about them as she did.

The mares could not believe nor understand what they were witnessing. Twilight Sparkle, their very close and dear friend, was, seemingly out of nowhere, removing the internal organs of an innocent fellow pony. Rarity's nausea was worsening till it was too much. Her stomach erupted and she vomited several times in a row, making a big, strong odor having, mess of bile all over her body and the floor. Dash violently thrash her body, so desperate to break free so she could rescue Pinkie. The light from her element was shining brighter than ever.

"Interestingly enough, Pinkie is so healthy. And considering how many sweets she consumes... yeah, I'll have to look into that. I'm guessing it's how she hops around a lot, using those muscles." Twilight said, briefly studying Pinkie's long intestine before folding it into a glass jar. Twilight dipped the bloody blade into a bowl of alcohol to clean it. After giving Pinkie's vulva a few good rubs, she used the scalpel to pierce the skin of the lying pony's groin. Pinkie could feel the very sharp tool easily dig deeper into her meat. The demented surgeon started carving out Pinkie's sensitive genitals, very slowly and carefully as not to damage any internal parts of the pink pony's sex organ. Pinkie's awareness faded.

Once finished, she lifted the blood dripping severed private part with caution. The vulva, vagina, cervix, uterus, fallopian tubes, and ovaries, all still connected. "Reproduction. Very important." Twilight poured alcohol onto Pinkie's disembodied marehood and placed the entire reproduction system into a vacant container and sealed it tight. She then spilled the alcohol onto Pinkie's genitaless crotch.

Noticing that pinkie was in unconscious state, she took out a hypodermic needle and injected Pinkie with a powerful adrenalin. Pinkie awoke with a gasp.

"Wake up Pink." Twilight said. "You don't want to miss your last lesson in life!" said Twilight as she prepared for the final incision. "I'll remove your lungs and heart after, so you can witness the lecture on your brain before I take it out."

Sad, confused, crying, and in tremendous pain, Pinkie lost the will to hope. She was just waiting for it to end. Twilight tried directing everyone's attention to the monitor. "Notice the high amounts of alpha brain waves on the left-hemisphere of Pinkie's brain, which indicate depression. Also there's an excessive amount of theta waves. These can be a sigh of depression and anxiety- ARE YOU ALL EVEN LISTENING?!"

Twilight applied her magic to force everyone's head to face the monitor. "Basically, her brain waves are showing the depression and anxiety she is feeling. Now then."

Pinkie's helmet was removed. A sharp painful pinching sensation was felt as the scalpel cut across the small pink horse's forehead and around the cranium. The wincing pony's forehead was oozing out red fluid. Twilight pealed off the flesh from the top of the head, taking a part of Pinkie Pie's poofy pink mane with it, causing her to loudly scream. "AAAAAHHHHH! AAAAAAAAAAHHHHHHH!"

Next, using the bone cutter, Twi sawed through the skull bone along the incisions of the skin. Making a complete circle around the top of the head, she lifted the head bone piece off, exposing Pinkie's tender brain. Pinkie braced herself as Twilight squirted more alcohol onto the brain. The sensation was that of an immense, heavy, pounding headache all over the surface of the her thinking organ. And Twilight caressing it with her hoof wasn't helping. But then Pinkie's awareness faded yet again, and was dangerously close to death. Dash saw this. The light from her jowl bearing necklace dimmed greatly.

Seeing this, Twilight noted, "Look everyone! At Dash's element! Pinkie is on the verge of death. The light is almost gone, because Dash cannot be loyal to someone who is dead."

Seeing an unconscious Pinkie, the purple unicorn directed her horn to the dying mare's head and zapped her with just enough magical life energy to keep her alive and awake for a little while longer. "That was a close one!"

Pinkie awoke. The purple unicorn applied her magic to levitate a kind of brainwave analyzing device to her. She put it up to Pink's head, "Isn't this interesting. The left temporal lobe is just as active as the right. I expected the left side to be far less active. Would you ever forgive me for taking you to be a moron Pinkie? Hee, hee, hheeee.." Pinkie was too weak and in too much agony to be mad, or even care about the cold words escaping from her tormenter's mouth.

Twilight continued. Taking a measuring tape, she measured the dimensions of the thinking organ. Rapping the tape around it and across it, vertically and horizontally, she jotted down the dimensions. "I'd tell you all the size of her brain, but it's not like you care about learning more about Pinkie. Maybe Rainbow Dash will be of more interest to you. Let's finish this experiment, shall we?"

The terrified mares knew what was coming. They could not bear to watch, yet they couldn't look away. Twilight Sparkle reached under Pinkie's ribs and took hold of one of the lungs. Streams of tears profusely flowed down from Dash's eyes, "This... *sniff*... isn't the Twilight Sparkle we knew... *sob*" Twilight hesitated for a moment... but then pulled on the lung until it disconnected from Pinkie. Everyone cried even harder. Pinkie, already struggling to breathe, found it much harder to take in oxygen with only one lung. "Nos" and "pleases" reverberated throughout the basement laboratory. Twilight placed both of her hooves on the only lung Pinkie had left. Twilight was hesitant again. She started sweating and breathing heavily. Using her magic to levitate her fizzing liquid filled laboratory flask up to her mouth... she took a chug of it, and then forced out the last means Pinkie had to breathe.

Within about four minutes Pinkie passed out from lack of oxygen. They knew it. Her friends knew that in a few moments the inevitable would happen to the blood covered pony. After a short passing of time, they saw the beating of Pinkie Pie's visible heart stop. Her eyes slowly closed. The brain activity ceased. They so desperately didn't want to face the ugly fact. But they had no choice. Their friend Pinkie Pie... was dead. And the glow from Dash's element of loyalty was completely gone.