Twilight's life is in danger as a secret organization aims to take the life of Celestia and Luna. After discovering the remains of the vampire Alucard, Twilight is the new leader of an old organization called Hellsing, originally established during the great war by Luna before she became Nightmare Moon. Now Twilight, the Elements of Harmony, and Alucard must face and defeat those who want the lives of the Princesses.