Trixie comes back to Ponyville to take revenge on Twilight Sparkle by robbing her of her innocence, but Trixie is herself captured by Rainbow Dash before she can fully finish the task.
She then has to endure the sexual woes brought on by Rainbow Dash, who is furious at the abuse of her friend, and a Twilight Sparkle, driven to anger and vengeance by Trixies actions towards her.