This is a work of total fiction, made entirely for entertainment purposes. No money is gained from the production of this prose.

Twilight magically tightened the leather straps around the Human's wrists and ankles. The female looked about, seemingly curious as to it's masters intentions. She hissed at the coldness of the steel slab she was laid on and struggled against the bonds, not liking the situation. She huffed and growled, but it was to late for her to muscle her way out of the situation.

"Sorry about this, Sunshine." Said Twilight. "Your super strength means nothing without the leverage to build up any energy." She commented offhandedly. Twilight spoke more to herself than the human. She couldn't understand a word, anyway. Finally done strapping Sunshine down, Twilight turned to the center of her basement where a Super 8 camera sat. Magically flipping the switch, Twilight spoke to the Camera.

"This is Twilight Sparkle. So far, all of my attempts to return sapience to the Human species have been unsuccessful. Despite these failures, I have learned much about these creatures. From my understanding so far, it's not that the Humans are unintelligent, it is that they cannot access their cognitive brain. For some reason, absolutely no synapse can make it's way into the frontal cortex of the Human brain. As we all know, the frontal cortex is the hub of Cognitive ability.

"I have developed a serum," Twilight levitated a glass vial with a sickly green substance. "That, theoretically, can force electrical impulses in the brain. An internal defibrillator, if you will. By focusing this electrical activity in the frontal cortex, it is my hope that with these new synapses, the frontal cortex will be able to be "jump started" and connect with the rest of the brain. For more information on this serum, see Lab Book number 8."

Twilight turned her back on the camera and made her way over to Sunshine, who had finally calmed down.

"If this doesn't work..." Twilight began. "Then I'm so sorry." She gently placed a kiss on the Sunshine's temple. Twilight took the vial and placed it in a steel syringe.

"This is test number one using the serum, and the Human named Sunshine is Experiment number Six-Two-Five. Subject is Twenty-one years old, female. One hundred sixty-five centimeters tall and one hundred twelve pounds. Fair skin, red hair and green eyes." Twilight magically wrapped a rubber band around Sunshine's arm, exposing a vein. "I am administering the serum now."

Slowly, the syringe floated over Sunshine's arm before piercing her skin. The plunger was magically pushed down and Twilight removed the syringe. Then she waited.

Sunshine began to whimper, her face reflecting discomfort. She shook her head, as though she were trying to shake a thought away. Her whimpers became squeaks and her squeaks became screams. Twilight could only watch.

Not understanding, Sunshine turned towards her only possible aid, and looked Twilight right in eye as she continued screaming. Slowly, blood began to leak out of her nose and ears. Nothing but pain could be detected by the poor human creature. Then her whole body began to shake violently. All the while, her screams never stopped.

Seizure... Thought Twilight.

Finally it all stopped. The seizure, the shaking, the yelling, the crying. Sunshine's body laid unmoving. Twilight approached slowly and rolled Sunshine's head over. Gasping, Twilight turned and bolted out of the basement.

The only thing watching Sunshine's body was the Super 8 camera. It looked right into her face which was twisted into the visage of agony. Her eyes had rolled back into her head and blood slowly dripped from them and her nose and ears.

Sunshine was no more.

A month had gone by since Experiment Six-Two-Five had passed on. Twilight had thrown herself into her work, hoping to forget. Well that was half true. Twilight wanted to forget, but she hardly threw herself into her work. After autopsying Sunshine, she saw that her brain was slowly cooked from the electrical impulses that her serum had placed there. So, she halfheartedly decided that her Serum worked too well, and cut it's potency by ninety percent. Same formula, same everything, just a severe lack of power.

She was tired. Over six hundred humans dead, all for her quest to bring them out of their feral hell. She didn't even remember why she got involved in this convoluted project in the first place. Intelligent humans were an old pony tale. They never existed!

That's what they said about Nightmare Moon... Her subconscious challenged. And King Sombra and the Crystal Empire. And the God of Chaos, Discord. And---

Twilight shook her head roughly.

"Arrggh! I get it!" She acquiesced.

"Twilight? Are you alright?" Asked Spike. He was in his apron, walking out of the kitchen with a stack of pancakes.

"Not really, Spike." She mumbled. Spike saw her depressed state and knew immediately what happened.

"Still thinking about Sunshine, huh?" He guessed. Twilight nodded. "Twilight, I know this might sound a little rough," he said gently. "But you need to get over this."

"How could you say that!?" Twilight yelled. "You didn't see what I saw, Spike! You didn't cause her suffering., I did!"

"I'm sorry that you had to go through that, Twilight. The fact of the matter, however, is that she's dead and the only thing you can do is learn from the failure. You've done this six hundred and four times before, and you've moved on. It's time for you to move on again. Take what you learned, and start again." He said.

Twilight thought about the serum. She had five new bottles of it in the refrigerator in the basement. Honestly, she could start again at any time. All she needed was a new subject.

"The serum started to work, Spike." Whispered Twilight. Spike looked up at her from his seat.

"What do you mean?" He asked.

"Humans don't display emotion. Not outside of anger, at least. Before things went wrong, Sunshine looked at me. I don't mean that she was looking at me, I mean...she looked at me, Spike. She couldn't do anything other than yell, but her eyes..." Twilight shivered. "She was begging me for help, Spike. She didn't want to die. I gave her the intelligence to understand she was going to die. I can't imagine how horrible it must have been for her, to understand what was happening."

"Twilight...if you want to stop, you can. You don't have to continue with this research." Spike said. "You've been doing this for years, you don't have to keep going."

That was true as well. This didn't have to be her life's work. She could do more with her life. Twilight shook her head.

"No Spike. One more. I owe it to Sunshine to at least try the Serum once more, especially if worked."

"Okay, Twilight. One more." He agreed.

The Apples weren't only the best Apple growers in all of Equestria, but they also had the best Human Farms in the country as well. If you wanted a highly trained human of any kind, there was nowhere better to go for one.

Coming up on the hill where Sweet Apple Acres was located, Twilight could see buildings coming into view. First the Apple Family home and barn. Then she saw the large grey building where all the humans were kept. Every so often, Twilight would see a human doing some odd job, or just resting in the shade of the large apple trees.

"Howdy, Twilight! What can I do you for?" Applejack called out with her typical southern twang.

"Hi, Applejack. I'm looking to buy a Human."

Applejack rubbed the brim of her hat with a hoof.

"'Nother one? Wat happened to old Sunshine?" Asked Applejack. Twilight knew she wasn't going to get over Sunshine anytime soon, so the tears springing to her eyes were expected.

"Sunshine...got into some live wires in my basement." Twilight sniffled. "She was electrocuted a month ago." It may not have been how it happened, but there was enough truth to get by Applejack's lie detection skills. Sensing only Twilight's sincere regret and grief, Applejack comforted her.

"C'mere, sugarcube." She wrapped her forelegs around Twilight. "So sorry to hear that, Twi."

"Thank you for your condolences, Applejack. Unfortunately, the work that I'm doing requires a human, so I have to get another one soon. I hope you don't think that's cold of me."

"Course I don't, sweetheart. I understand perfectly. It's just bad luck that old Sunshine...went away." She said gently.

After a few minutes of comfort, Twilight removed herself from Applejack.

"So, Applejack," Twilight gave a halfhearted smile. "Got any specials going on right now?" Applejack smiled, then it quickly became a frown. Hearing a commotion behind her, Twilight followed Applejack's gaze behind her and saw a conglomeration of humans surrounding what looked like...a fight.

"Damn it, not again." Bolting in their direction, Twilight watched as Applejack ran into a crowd of excited humans. Nervous for her friend, she followed after her.

From inside the group of Humans, Applejack let out a piercing whistle which gathered all of their attention.

"HEEL!" She cried. The effect was instantaneous. Every human in the vicinity immediately sank to their knees and bowed their heads. Looking at the fighters, Applejack shook her head. "Poor Orion."

"Applejack! What happened!?" Asked Twilight.

"Just a stupid fight." She responded. "Can you do me a favor and carry these two over to the Habitats?" Applejack gestured to two male humans. Applejack herself was herding one bloody male on her own. Using her magic to lift the both of them, Twilight and Applejack made their way to the Habitat. The rest of the humans scattered.

"You were asking earlier about any deals? I'll give you Orion, here, for half price." Said Applejack "Nothing but trouble follows him around." She huffed.

"I don't think I have much use for an undisciplined---"

"Undisciplined!? Orion here is one of the most behaved humans around!" Applejack corrected.

"Then why does trouble follow him around?"

"Orion here is a budding Alpha Male. Seems other males around don't like him cause all the womenfolk follow him around." Looking up at him, Twilight saw what Applejack was talking about.

Orion was a tall human. One hundred and eighty centimeters tall. His body was fit and muscular. All classic signs of a human Alpha Male. Looking up at his face, she saw strong masculine features, strong, squared jaw, angular face. His brown, messy, medium length hair shone in the light and bounced with every step he took. His deep brown eyes shone in the sunlight... Twilight could easily see why the female humans would seek him out.

"Poor Orion will just be minding his own business, them WHAM! Some other male will start beating on him because his woman was looking at Orion instead of him. Only sixteen and he's been in more fights than any two humans combined." Applejack shook her head sadly.

Locking up the two offending humans in the cages, Twilight and Applejack led Orion to the infirmary.

"Can he follow basic commands?" Asked Twilight. Applejack started cleaning up Orion's wounds.

"Yup. He's all set for adoption, if you want him. He needs to get away from such a huge number of males. To them, he's a threat. Half-price, Twilight. Take him Twilight. Give him a safe place to live in." She asked.

Safe place? There's a chance I'm going to kill him. Twilight thought to herself sarcastically. But, he was being offered at half price. Twilight nodded.

"Alright. I'll take him off your hands, Applejack. I promise I'll take good care of him." She said.

Applejack grinned.

"You hear that Orion? You're getting yourself a home!"

Twilight magically tightened the leather straps around Orion's wrists and ankles. The human looked about, seemingly curious as to it's masters intentions. He hissed at the coldness of the steel slab he was laid on and struggled against the bonds, not liking the situation. He huffed and growled, but it was to late for him to muscle his way out of the situation.

"Sorry about this, Orion." Said Twilight. "Your super strength means nothing without the leverage to build up any energy." She commented offhandedly. Twilight spoke more to herself than the human. He couldn't understand a word, anyway. Finally done strapping Orion down, Twilight turned to the center of her basement where a Super 8 camera sat. Magically flipping the switch, Twilight spoke to the Camera.

"This is Twilight Sparkle. My last attempt with my serum...did not end well. After performing an autopsy, I was able to determine that the serum had partial success. The frontal cortex was beginning to...activate. Unfortunately, I never set up a fail-safe for the serum, and experiment six-two-five was essentially...electrocuted from the inside-out."

Twilight looked at the camera, recalling the aforementioned events with a shiver.

"However, I have modified the serum. This serum has been altered to only use ten percent of it's original power, while maintaining it's original objective."

Twilight turned and stepped towards Orion, who was still struggling in his bonds. Magically tying a rubber band around his muscular arm, a vein was exposed.

"I hope this works, otherwise there's going to be a lot of disappointed women out there." Twilight tried to joke. "Please be safe, Orion." She kissed his temple.

"This is test number two using the serum, test number one using the Serum two-point-oh. The Human named Orion is Experiment number Six-Two-Six. Subject is sixteen years old, male. One hundred eighty centimeters tall and one hundred ninety-seven pounds. Tan skin, brown hair and brown eyes.I am administering the serum now." Once again, the syringe full of the serum was stuck into the human's arm, and the plunger was pushed down. Removing the needle and stepping away, Twilight simply observed.

It began the same way. Orion slowly began to become aware of an uncomfortable feeling in his head. Logically he began to shake it, hard. Then the pain came, and he could only scream. He screamed from his very soul, and he turned to look at Twilight. Bleeding from the nose, ears and...eyes. Looks like another failure.

Suddenly, his body jerked and began to shake violently.

The seizure... She mentally checked off.

Finally, it all stopped. Orion's body laid still on the steel table and Twilight began to approach it. His head laid in a pool of blood, and his skin was flushed, but otherwise...he looked dead.

Twilight managed to choke down a sob. That was it. Total failure. Years of research, all down the drain. She was such a fool.

"AAARRRGGGHH!"

A roaring scream scared Twilight from her funk and she looked down into Orion's face. His eyes were open. Bloodshot, but still open.

"STOP! Stop! Stopstopstop---MAMA! Mamamamamamamama..." He cried until he couldn't anymore.

Too exhausted and too traumatized, he slowly fell back into unconsciousness.

Twilight couldn't believe it. Orion survived...and he spoke! He knew he wanted something...and he was intelligent enough to verbalize it! She had succeeded!

Twilight Sparkle had made a Human Being sapient!

Author's Note

I've taken some liberties with the Your Human and Youverse. Nothing really changed, just some extra info and a few tidbits here and there.
-Humans are physical Juggernauts. Like earth ponies x10

Yes, Experiment 626 is a reference to Stitch, from Lilo and Stitch.

I figure, like humans can tell if an animal is attractive to other animals, than ponies would be able to identify traits that make humans physically attracted to each other.

Why can Orion speak? He can't. Like animals can learn human language and associate it, Orion has learned a few words and associations. He only used 2 words. One was Stop, which he has heard enough times and is now intelligent enough to know what it means. The other was Mama, which he now nows means mother. He was asking for his mother.