It's been fourteen years since the Cutie Mark Crusaders got their cutie marks. After that, they went about their normal lives. Apple Bloom runs a hardware store in Ponyville, Scootaloo joined the ranks of the Wonderbolts, and Sweetie Belle is now a recording artist under Neon Lights' record label.

But a new threat has come to Equestria, and this time, it's up to these three mares, along with Babs Seed, Twist, and Dinky Hooves to protect everypony, for they're the new Elements of Harmony.