In a world where Celestia and Luna are merely stories, Equestria is filled with murder and intolerance. A young Twilight Sparkle serves as the newly crowned Queen, but her many responsibilities stop her from doing much for the people. To assist the Royal family with this problem, an organization called Hellsing was created many generations ago.

Rarity, an independent and strong young woman, is the head of the Hellsing organization. Alushy is her vampire servant whom she uses as their weapon to defeat the occult. Together, Alushy and her fledgling, Pinkie Pie, must defend Equestria from vampires, ghouls, and the rising forces of Major Moon.