Discord tries to prove his throne worth, Rainbow Dash eager to get closer to Daring Do. How does this add up. Tirek is on the loose again for revenge, and he makes the most unusual team-up with another villain, and that's DARING DO'S ENEMY AHUIZOTL!

Fido and Spot's friend Rover is also captured by the two giant monsters, and Discord has trouble confronting his fears after his traumatizing past involving Tirek. However, Twilight and her friends enlist him and the dogs to come help, so they could all learn to face their fears into the deepest dangers of the jungles ahead of them. Rainbow also convinces Daring Do to guide them to Tirek and Ahuizotl, even if she'll act to hyper around her. The gang of eleven will face deadly puzzles, death traps, giant monsters, and unexpected strength from their enemies that they aren't prepared for. It's the biggest team adventure yet, and quite possibly, the biggest teamwork involving Rainbow Dash and Daring Do once more.