On the way to a vacation in Manehattan, Applejack's car breaks down just outside of Canterlot, forcing her, Twilight, Pinkie, Rainbow, Rarity and Fluttershy to seek refuge inside an abandoned old mansion on top of the hill overlooking town that is known simply as "The House on the Hill." However, as soon as they walk through the front entrance, the door suddenly shuts and locks itself behind them. With no way to signal for help, Rainbow suggests searching the house for another way out. And so, with everyone equipped with a flashlight and walkie-talkie, they split up and begin to search the house. Little do they know that the mansion hides a terrible secret; one that just might compel one of the girls to make an attempt on the lives of her "friends."

Based on the tabletop RPG "Betrayal at House on the Hill."