Ponyville is in turmoil. Princess Twilight Sparkle has simply vanished without a trace, leaving her friends scouring the land in search for her. Meanwhile, Ponyville hospital has just received a very peculiar patient of unknown origins… Could the two events possibly be linked?

And while the ponies are worried about their missing princess, somewhere far away, a vile creature plots its revenge against those who have deprived it of its chance to have a happy family.