Once upon a time, in the magical land of Equestria, there were two regal sisters who ruled together and created harmony for all the land. To do this, the eldest used her alicorn powers to raise the sun at dawn; the younger brought out the moon to begin the night. Thus, the two sisters maintained balance for their kingdom and their subjects, earth ponies, pegasi, and unicorns alike.

But as time went on, the elder sister became obsessed with power. The ponies relished and played in her day, but the night, as beautiful as it was, seemed to mean nothing to the ponies. One fateful night, the elder alicorn refused to lower the sun to make way for the dusk. The younger sister tried to reason with her, but power had corrupted the elder's heart and had transformed her into a wicked mare of fire: Daybreaker.

She vowed that she would illuminate the land in eternal light. Reluctantly, the younger sister harnessed the most powerful magic known to ponykind: the Elements of Harmony. Using the magic of the Elements of Harmony, she defeated her elder sister, and banished her permanently in the moon. The younger sister took on responsibility for both sun and moon, and harmony has been maintained in Equestria for generations since.

When Luna tells her faithful protege about the upcoming return of Daybreaker, Trixie Lulamoon is tasked to travel to Ponyville to find the new wielders of the Elements of Harmony. But how will she find them when she doesn't even know who they are?

But Trixie's troubles aren't as simple as finding the Element Wielders, as a group of resistors against Luna are preparing for something...

This series isn't specifically based on the RainbowDoubleDash's Lunaverse, but some inspiration was taken. This is the first episode of The Alterverse.