Twilight's Science Class

by FruitTrifle

Rating: mature
Tags: Gore, Porn, My Little Pony: Friendship is Magic, Pie Sisters, Fetish, Comedy, Sex, Horror, Profanity, Non-consensual, Big Macintosh, Death, Main 6
Length: 16344

Twilight does science, the best way-with friends!

Twilight, disappointed with the materials available on pony anatomy, recruits her friends to right this!

It's gonna get weird

Unsafe science practices, death, beginning of spiraling madness, sadism, torture, Food Science, Helpful Victims, Death, Futa, Deathgasm, Exercise Science, Death Grater,Necrophilia, Rape, Crushing Science, Bone Breakage, Amputation, Incest, Excessive Cums for Science, Mind Control, Forced Corpsy Cunnilingus, Forced Necro, Slight Dismemberment, Acid science, sex science

Published 2019-10-15 10:20:01 UTC
Last updated 2023-02-08 12:27:24 UTC

1 Twilight Pursues Knowledge

Twilight finished shelving her books, satisfied her library was in order, no books missing. Her blissful revere was popped by a knock, Fluttershy peeking her head around the door.

“Um, sorry to bother you, Twilight...but, well, I need to borrow a book on pony anatomy.”

“Sure thing! What kind of pony?”

“Pegasus, please,"

Twilight nodded, looking over her collection. “Hmmm...most of these aren’t very in-depth... sorry, Fluttershy, I don’t have reason to keep a medical level book here,”

Well, that wasn’t entirely true-she was very curious, but it was frowned upon to have that sort of gory book in your possession if it wasn’t tied to your work or cutiemark. And as a princess, she had to be careful not to embarrass herself, or the others.

She tapped a hoof to her mouth, thinking. Perhaps she could...write it herself. Then they couldn’t trace a book like that to her-and surely her friend’s would help, and if she HAD to look...closer, she could heal them right up again!

She turned to Fluttershy, beaming, “Let’s MAKE one! We’ll do science, and record it in a book! It can be a group effort, like the journal!”

“O-oh, you...really think I can help?”

“Of course, Fluttershy! Everyone can! We’ll just start with you, since you’re here, and we’ll do Pegasus anatomy first! Follow me!”

They trotted out of the library, and down the stairs of the castle into the lower floors. There were many, admittedly, but it didn’t take long to get to her lab. Twilight sent Fluttershy to sit in a chair by a table, as the Pegasus marveled at the high ceiling above them.

“Goodness...why is that so high?”

“Hm? Oh! Ventilation, in case chemicals go off, or wrong, or some such. The longer it takes the room to fill, the faster I can notice!”

“Oh dear...is...that going to be an issue today?”

“No, not at all! We’re doing PRACTICAL science, no chemicals involved!” she assured the shy mare. She went back to rummaging. She pulled out a blank book, quills and ink, humming as she set them out on the table with her magic. She added many medical items for measuring and checking, blushing and trying not to pay too much attention to a speculum.

“N-now, let’s start with...measuring things, and weighing!” she got to work. It wasn’t very interesting to others, but Twilight was fascinated by it all-Fluttershy even let her have a feather she preened free for study.

“Oooh, I can’t wait to compare this to other pegasi-or even other ponies in general!” she enthused, Fluttershy smiling at her friend’s happiness. Twilight took a few small fur and mane samples too-she made sure to take them from places that wouldn’t show in a rare show of understanding.

“Well, now, can you fly a few loops around the ceiling? I want to write down some observations on the flight,” Twilight requested, her friend nodding and taking off. Fluttershy flew in circles, and figures of eights when asked. So into making her friend happy was she, that she didn’t notice the light in front of her. Her mane covered up much of the illumination it made until it was too late, and the pegasus crashed into it. It blew, breaking, Fluttershy screaming as glass peppered her, and she plummeted through the air.

Twilight ran forward to help, but was too late. Fluttershy smashed into the ground before the princess could do more than slow her slightly with magic-and judging by how the pegasus gagged and coughed up blood, that hadn’t been a mercy. Twilight looked at her, full of sadness-she couldn’t fix this. It was too much, too deep inside, for her magic to fix.

She sat next to her, stroking her hair. “Oh, Fluttershy, I’m...I’m so sorry...”

“I-I….it’s okay, t...twilight, I...I wasn’t careful.” her breath rasped through blood and bone in her lungs.

Twilight nuzzled her, before raising her head. “...Fluttershy. I...I’m sorry, but...w...when you...” she sobbed, before pulling herself together. “Can I...keep going with the book? Both with our friends, and...and your...body,” she managed, sniffling.

Fluttershy nodded. “Y-yes,my...animal friends have...helped me...appreciate using all of...a body, and if I...can help you in death then...I will,” she ended it with a heavy cough, blood spraying out of her mouth. Twilight sniffled, nuzzling her.

“I’ll be here with you, till the end.”

“It-it’s okay, I already...feel numb...” this made Twilight perk up, and she carefully lifted Fluttershy’s broken body into the air with her magic, putting her on the table. “I’m going to start now, Fluttershy-I’m sorry but I can learn a lot while the body is still alive,”

She nodded, “That’s f-fine, Twilight,” she smiled faintly. Twilight did dope her up-just in case she did end up feeling something. She used a scalpel, slowly cutting the other open from neck to navel, using her magic to crack the rib cage open, and push out like reverse wings.

She took notes, and kept an eye on Fluttershy to see when she passed. She also held hooves whenever she could, not wanting her friend to feel lonely during this frightening time. She was careful with the pegasus’ organs, not tugging too hard, or often, to keep her comfortable. It might not hurt, but she was concerned it might be unpleasant to feel.

Fluttershy looked to Twilight, “I...I feel it. I’m going...I...I love all of you girls, let them...know that, please...if it’s….not...too...much….t...t...” she sighed, lungs going limp as her heart stilled. Twilight held her hoof throughout, crying and nodding. Of course-of course she would.

She rubbed her fur of tears, and got back to work. She glanced down to the nethers, now the owner was gone, and blushed heavily-her muscles were still...working, a little.

...what might that feel like, when-

She shook her head violently. No. Absolutely not. She couldn’t think like that-she was a princess! It was a slippery slope, as Luna had once found. She wouldn’t end up becoming...Midnight Sparkle, or something else just as ‘edgy’.

She began to pace. Then again...there was no real documentation on a pony’s last moments, including...muscle spasms...she sighed, knowing this was no more than rationing from what she now suspected was a diseased mind.

Author's Note

I know this might get a lot of downvotes, and i'm fine with that, it's not everyone's cup of tea. I am open to feedback, however.

2 Twilight & Rarity Share a Secret

Twilight sat sadly in front of her castle’s furnace, her friend’s body burning. She rubbed a hoof across her muzzle-she wanted to give Fluttershy a proper buriel, but even if the princesses pardoned her, the trial would ruin Twilight forever. And then...there was the other issue…

Her gaze wandered to the stairs. She wanted to do more science. She wanted to write more on the workings of ponies...but most of all...she wanted to feel that tingling in her nethers as she did so. Was it just the cutting open of the ponies? Was it the life, held in her hooves? Was it the pain they might die in?

She was appalled to realise, when she looked back, she was upset at how little Fluttershy had suffered. She got up, pacing. This...this had awoken something in her. She was afraid. She was exhilarated. She was...aroused.

She went up the stairs slowly, deep in thought. Well...one of the elements was dead...so it didn’t matter if more were...so what if she, just...kept experimenting on her friends? Looked at how the three pony tribes varied.

She came back to her senses in the foyer, mulling it over again, when Rarity trotted in.

“Oh, darling! Have you seen Fluttershy? She was meant to met me at the spa after getting that book she wanted?”

Twilight went pale under her fur. A witness...well. The choice was made for her.

She faced Rarity, and sent a bolt of magic at the other’s horn, leaving a crack from base to tip. Rarity convulsed, screaming, and passed out from the massive pain of horn trauma. Twilight lifted her in her magic, carrying the unconscious unicorn down the stairs to her lab.

She laid out the dress horse on the same table where Fluttershy had passed, using leather straps to keep her still. She prepared her tools, taking long enough Rarity awoke, sobbing immediately.

“It hurts, why does I-w...wah...where am I? Why am I tied do-Twilight! Please, release me, we have to report whomever has done this!”

“It was me, Rarity...I’m sorry, but I need to...to get off to this! I mean...science! Yes, science!” When she turned to collect her tools, Rarity could see just how much of a lie the latter was. The princess’ tail was hiked up, fun goo drooling profusely down her thighs, like she was in heat.

“T...twilight, please...we can...talk about this surely? I...” she thought hard, but there was little she could bribe a princess who lived in a crystal castle with. “I can help?”

“Don’t be silly, partners increase the danger of being caught, and I don’t plan on getting caught. I’ve thought of alibis for all six of you! Seven, if I can get my hooves on that fake magician Trixie...” she grumbled.

“...dear Celestia, are...are you...going to do this to everyone?!”

“Of course I am...and then maybe my self, too-it’ll be hard to pull myself apart AND record it all, but I believe in myself!”

“Twilight, enough! Listen to the madness you’re spouting!”

“...you’re right, it is madness...and it...feels so good! No wonder Discord wanted us all to feel this, it’s amazing!” She pranced over to Rarity, a chisel and hammer in her magic, as well as a journal and everything needed to write in it.

“Now, please be open and vocal about how it feels, this is for science!” she positioned the chisel at the tip, “I’ll take it piece by piece, and record the bits and what it looks like inside! This will give me great data on traumatic horn damage as well,”

“...Twilight...Twilight please, no...I don’t want this!”

Twilight huffed, “Well, that is hardly generous, Rarity!” Somehow...Rarity felt she was right- she should be more generous, and what higher generosity than giving her life so generations of ponies would know more, and not have to under go such pain?

“...very well-just please forgive me if I cannot keep a lady like composure.”

“Of course I will!” she stroked her friend’s mane with a hoof comfortingly. “And as painful as this will be, I won’t hold it against you,” she promised, before replacing the chisel, and giving it a solid whack.

Rarity screeched with such pain and horror Twilight wondered if she’d turned the poor mare feral, before Rarity begged to be let out of her pain. She seemed to come back to herself, shaking her head- or trying to before the jolted tool in her horn hurt far too much. Twilight made notes, asking questions and writing as Rarity as best she could through tears and agony.

Twilight struck the horn again, and it cracked right off, in many pieces. Rarity’s eyes rolled back in her head, and no matter what Twilight tried, she couldn’t bring Rarity back to her senses. Not shocking, not smelling salts, nothing. The closest she got to a reaction was when she poked the horn’s remains. This intrigued Twilight, and she got a few more tools.

She sat on Rarity’s barrel, blushing heavily, but the table was too small for much else. Book held in magic, she made notes in it again as she placed a nail in the horn stump, carefully hammering it in. Rarity jerked, Twilight rubbing against her before stilling her hips with a fair amount of shame. She cast an electricity spell at the nail, watching Rarity convulse, making notes on it.

She sighed-interesting, but not all that helpful. She got down from the table, pacing as she thought deeply. “Hmm...still, this is good...oh, of course!” she began vivisecting the unicorn as she had Fluttershy, pulling her apart, making notes and drawings. Unicorns seemed lithe, but not as light as Pegasus ponies-most likely their inherent magic for flight.

“I wonder how it compares to earth ponies...” she mused, as Pinkie popped out in typical Pinkie fashion from the doorway- covering the room in confetti.

“Heya, Twilight! I- oooooh, we’re doing THAT sort of fic, huh?”

“...I...what?”

“Well that’s cool with me! Can I request a science?”

“...yes?”

“I wanna die the way I lived- eating waaay too much! Feed me till I pop, Twilight!”

3 Twilight Does a Party Science

“So, you want me to...feed you...until you literally pop?” Twilight asked slowly, making sure she was getting this right.

“Yup! Sure do! I know you’re doing a Science, so, think of it as stress testing an Earth pony, from the inside out! And you can do AJ from the outside in!”

“Huh...that...makes sense...I’m proud of you, Pinkie- not only so open minded, but science minded too! Well, let me clean up, and-”

“And I’ll make the food! It’ll be easier if I do it than you having to buy it or something,”

“That’s very thoughtful, thank you Pinkie-the kitchen is all yours,”

Twilight went to her lab, happily setting up a gyno chair with restraints, so she could see Pinkies stomach the best. She’d be able to measure too! It was a shame she couldn’t weigh as well, but weighing the food first and comparing would be enough. She picked up another notebook, the first one now full of illustrations and science.

She giggled, prancing about, excited about it all. She couldn't help the goo dripping down her thighs, which did shame her...well, if anyone could forgive her for being too excited at something, it was Pinkie.

Pinkie came tumbling down the stairs atop a wave of food. "Okie dokie lokie! I've got all the food, Twilight!"

She climbed into the chair, just as wet as Twilight, and let herself be strapped in. “Okay Pinkie, as you requested, I am going to feed you the sweets you made until you literally burst. Please, keep all remarks to how it makes you feel, okay? It’s important to note that down!”

Pinkie nodded eagerly, her pink head a blur. “Got it, Twilight! I’ll let you know how it feels as you stuff food down me!” she promised.

Twilight started out small, cupcakes with icing, Pinkie eating them happily. Twilight had a feeling that might change, even for the party slut. They got to two dozen, the earth pony still going hard. They hit five dozen and three cakes, and she began to slow down, looking green.

“Urgh...Twilight...feel sick...gonna...but don’t wanna...”

Twilight cast a spell. “There-now you won’t bring anything back up,” Pinkie managed a relieved smile, and they continued.

Three more cakes. Six. Ten. Hay fries by the literal magic ethereal shovel, poured down her throat. Burgers, three at a time, although Twi did eat one or two herself. By now, Pinkie’s stomach was huge, bigger than even a heavily pregnant mare. Twilight rubbed a hoof over it gently, feeling the tension in it as Pinkie whimpered, no longer able to speak with the pressure on her lungs, and on her throat from the bottom.

Twilight levitated a muffin to be the last one, as Pinkie screamed, her stomach tearing suddenly from the sides up. Half digested food blasted out, Twilight bringing up a shield to protect her from the gore and mush. Once the screams were gurgles, she lowered it and trotted to her friend. Pinkie gasped for breath that her squished, flat lungs no longer gave, her eyes unfocused.

Twilight licked her lips. Oh, so wrong...but so hot!

She cast a spell, giving herself a stallion’s equipment under her tail, and mounted the still spread Pinkie. Pinkie managed a smile, glad she’d give one last smile before she’d go. Twilight’s equipment was hard as soon as it came into being, too excited to stay down at all. She unleashed a flurry of thrusts deep into Pinkie’s cum sucker, panting, one hoof against the other’s heart so she’d feel it when she died.

Pinkie, ever the good sport, actually rolled her tunnel up and down the horse cock stuffing her to pleasure Twilight. Twilight snuggled her in response, the mares exchanging an admittedly bloody, sickeningly sweet kiss as they made love. It ended as Pinkie wheezed, eyes rolling back and body seizing. Twilight felt it as the earth pony’s heart stuttered to a halt, Pinkie’s body spasming even harder than it had all evening. Twilight blew a load into her cunt, the cum splattering out the torn womb and over her exploded insides.

Twilight didn’t even rest before ramming her cock into Pinkie’s dead tail hole, now committing necrophilia. She didn’t care, it felt so good! She suddenly understood how others became evil, giving in to their urges. Not as extreme as this...maybe Nightmare was, but it hardly mattered. All that mattered to her right now, and forever more, was her science and how hot it made her. She was sad she’d cremated Fluttershy before she could feel her dead holes, but Rainbow was on the list, and she still had Rarity to mount.

She at least had enough presence of mind to write down how an earth pony’s body felt during, and after death-she made sure to be detailed about movements and feeling. This was science, after all, she had to be thorough so she could replicate it later on! For...proper testing, of course.

She blew another load up the dead plot, joining the rest of her cum in the soup. She panted, laying on Pinkie’s body. She wasn’t used to so much exercise...speaking of. She levitated her book to her. She was eager to see how Rainbow Dash compared to Fluttershy, and had just the idea to get her to the lab. ...after cleaning, of course.

Cleaning...she looked around her, ear going flat. By Celestia...had she really done this? To her friends, for science, maybe but killing them...and the afterwards...pleasuring herself with cold, sticky corpse holes... Pinkie had been happy to do it. Fluttershy an accident...but Rarity! Dying in such a way...in so much pain...b-but the data on the pain! She began trotting back and forth, her usual nervous tick. M-maybe a painkiller? Less painful experiments?

She shivered, before collapsing and puking in the mess already there. She spat, heaving great sobs as she began to cry. Scared...she was so scared. Because of what she'd done...and knowing she'd do more. She wanted to, as much as she didn't. There was no going back...she got up, unsteady. She'd keep going...if nothing else, she'd finish her work. She had to. It couldn't be all for nothing.

4 Twilight Learns About Athletes

Twilight knew how to get Rainbow Dash to her lab-it was simple. Appeal to her pride, simply asking her to come for physical testing as a 'prime pegasus specimen.' As much as Rainbow's pride had improved to not be a huge hazard, that would still stroke her enough to turn up.

Twilight trotted around her lab to set up her device-a pegasus device, if you will. What was essentially a bondage harness hung from the ceiling, magically enchanted to read vitals...and keep the wings strapped down, comfortably at least. Under it, was a simple treadmill, Twilight planning on asking Rainbow to run on it. She knew even Rainbow would be suspicious of asking a pegasus to run, so planned on telling her it was simply to gather ALL data she could. Which was technically true, anyway.

She levitated more notebooks and quills and ink, making sure she had enough as well as spares, just in case. She had planned for everything in her anxiety...except one.

Rainbow came flying down the stairs to the lab as asked, but Applejack trotted after her. "Hay egghead! I told AJ about this, and she wanted to come see! And participate, not that she'll be as awesome as me!"

Twilight looked crestfallen, "Aw, Rainbow...I didn't want too many to know about this yet!" "Hahah, oh twi. Want whatever you're doing to be perfect first, huh?" "Well....yes...but it's okay! I did want to ask AJ eventually too, to represent earth ponies! Would you?" she asked.

AJ looked at the contraption, "Well I can't rightly say I understand what this is or why-" "To record an accurate and recent assessment of pony types!" "Uh...oh! Like how we record new and old apple variations?" "Exactly! Now, to start with...you both need to run on the treadmill," she explained her reasoning behind it, both agreeing.

They got strapped in, Twilight standing in front of a screen to the side of them. She used magic to manipulate it, the treadmill beginning at a walk. "I know it's slow, but I need to record how your vitals increase as the speed does-don't worry, I know you fillies want it fast, and it'll get there!" she assured them, the pair seeming happy with that.

As promised, the treadmill picked up slowly, and in a bit they were both running. "Alright girls, here comes the sprint!" Twilight warned, as it did indeed pick up to an all out sprint. Rainbow was struggling already, despite rather good endurance. "H-hay, Twi...I gotta..." "Oh, Rainbow..."

Twilight pressed a button on the screen, doors opening in the floor, revealing an angled downward cheese-grater, apparently, at a huge size. Pony sized, even. "That's disappointing. But I'll get all the data I need and more, your death won't be in vain!" "Wha....TWI THIS ISN'T FUNNY! WE ALREADY DEALT WITH UNFUNNY PRANKS!" "Science isn't meant to be funny!" She admonished. "Now. The speed you're at, if you slip, you'll be catapulted into this special metal board. You'll be sliced a fair amount, and I'll see how you react to stress in various ways! The same does apply to you, Applejack, but I expect someone with your physical work and stature to last much longer than a career flyer. ...I'm sorry. I know...I know this is scary-i need that data too-but the numbing agents on the board mean you won't feel it for long. Just...long enough. I really am sorry-but this...this needs to be done." It sounded more like she was convincing herself than her helpless friends.

Rainbow gritted her teeth, crying in pain and fear. Applejack looked at her, terror for her friend in her eyes. She gritted her teeth, looking forward, and leapt. She planned on saving Rainbow and stopping Twilight, but this was a possibility she'd seen. As soon as AJ leapt, the harness yanked her into the air. Her strong legs meant nothing in open air with nothing to brace or kick against. Rainbow wailed, knowing she wasn't strong enough to leap like that, even if the harness wouldn't yank her up.

"Oh Applejack...Even now you want to save a friend...But I have a special idea in mind for you," she smiled wetly. "You'll both be a part of science! Isn't that wonderful? All our friends will!" "Y-yall better not touch Applebloom or I swear-" "Of course not. A minor can't be considered for this sort of science. Besides, Scootaloo is of much more interest with her underdeveloped wings. I always wondered what did that, the poor dove." "P-plee-eh-heee-eze! Don't h-hurt Sco..."

Twilight tapped her hoof, before sighing. "Alright, I'll give you that last wish. I will not use Scootaloo for science, pinkie promise." Twilight wasn't sure if she could have done that anyway, not like she was doing to her friends. Rainbow sobbed in relief, but it was too much. She stumbled, hitting the tread and being flung back and down the grater, screaming as the grooves tore flesh and muscle from her body. Blood sprayed out from her body as she scrambled to climb up or even just halt her ripping journey down. She finally came to a stop at the end, wheezing in pain.

Rainbows hooves, back and front, were worn down to nubs at her leg bones, her chest skinned down to rib cage and sternum. In fact, most of her skin on her front was in tatters. The harness had survived, if just barely. Twilight raised her out of it, the harness lasting just enough to get her out of the range as the doors closed above the grater.

She smiled, even as the harness snapped, dumping Rainbow on her front, who howled in agony, her limbs convulsing and adding to her misery. Twilight strolled over to her, leaning down. "You know, I keep saying it's for science, and it is! But...I can't deny...how wet this makes me, to basically betray you and use you...make you hurt, die...I think I might be sick. Possessed, even...I know I should get help. Stop myself from hurting others, but I just...I need to finish this, and...seeing you like this...it's so...erotic. No one but AJ and I have seen so much of you, and she can't see much from where she is..." she whispered, casting a spell and growing a heavy sheath and balls with a shiver.

Rainbow looked confused, then horrified as Twilight rubbed her chubbing sheath against Rainbow's rear end. "Mmmh...You'll numb soon, so you...don't mind me having my fun, do you?" Rainbow writhed, "I do mind! Stop it!" She tried to kick her off, only to cry out as the spasming hurt her shredded parts. It was going numb, at least, and part of Rainbow marveled at how much more that would have hurt without it.

Twilight's mare meat spilled out, right into Rainbow's meat wallet. Twi churred, going to the hilt. "Fuck, Rainbow...so good...so tight, so hot..." She rolled her hips, leaning over to kiss her tenderly, a long, deep kiss. "I love you...I love all of my friends...I love having this last, romantic moment with you all..." Rainbow just gasped in reply, eyes dulling slowly. Even if she could respond, what could she say to that?

Twilight fucked her harder, reveling in the starting death spasms. "R-rainbow..." it was somehow erotic and romantic. No one else would ever feel this-her last moments, making love as she passed. She rolled Dash over, carefully, despite knowing the numbing agent and being so close to death meant Dash would feel next to nothing. She could SEE her cock stretching out the tunnel, hitting the cervix...it was too much.

She came, bloating up the dead womb with the magisperm, visible through the skin.

She pulled out with a sigh, cum dripping down RD's thighs. Twilight looked up at AJ, smiling, almost apologetic. "Now...I have an idea for you,"

5 Twilight Does More Case Studies

AJ's hooves ran uselessly in midair in her harness, sobbing out love for her fallen friend as her other, mad friend cleaned up the blood and cum. Twilight ignored her, humming as she made meticulous notes and cleaned. She lowered AJ, making the earth pony bellow in rage, finally able to get traction to charge at the alicorn.

The overpowered pony simply summoned magichains, locking Applejack in place, Twilight making note of the surprising amount of magic needed to hold AJ's natural strength. She bucked and snorted, but the chains kept her more or less in the same place.

"Now, now, Applejack! This is important-science, for the betterment of all ponykind! Don't you WANT to further knowledge, to help future generations know their limits?"

"Not like this, ya crazy pile a horseapples!" "Ah! LANGUAGE, Applejack! ...well, with all the gore and sex, I guess I can't be mad at some profanity." she admitted, scratching her head with a hoof.

She levitated over some heavy looking wooden squares, large and flat but thick. "...also, I promise-Applebloom won't be a part of this, it's the least i can do for you." AJ seemed to calm a bit at that. "...thanks, Twi...sorry fer the name callin'." "Oh it's okay! I know how you get about family, it's inspiring really!"

She got the squares in position over her friend's back. "Now...I'm going to test your resilience. I did it with Pinkie too, but with overfeeding. So, instead of from the inside, it'll be the outside!" "...ya know, if it weren't fer the fact ah die at the end, this might be interestin' instead a terrifyin'," "I know, Aj. It's scary... But you'll be memorialized in science! A contributor to furthering the understanding of the earth pony body!"

"...I ain't one fer science, so can we..." she took a deep breath, the alicorn nodding. "Of course." she lowered the squares slowly, letting AJ take the full weight before carefully releasing them. AJ stood. This wasn't so bad...so far, anyway. Twilight took notes, watching. After half an hour, AJ began to shift uncomfortably.

After an hour, her legs began to tremble. The blocks felt as if they got heavier and heavier, her legs bending under the strain. Twilight paid more attention, her futa cock at full mast again. She stroked it with her hooves as she did the notes in her magic. She stopped when AJ gave her a disapproving look. "S-sorry, no more for now."

"T...twi...it's gettin' too heavy..." "Do your best, Applejack! I believe in you!" AJ grunted, annoyed at the friendship bullshit at a time like this. Finally, with a grunt an hour later, her legs gave out, one breaking and the bone ripping through the flesh. She screamed, struggling under the wood. Twilight began to levitate more wood over. "Twilight...wh...what..." "Well, now i know how long you can stand with weight, I'm going to place more on you to see how long your body holds out, like with Pinkie!" She went behind Applejack. "And, well..." something poked the farm pony. "I'm pent up..." she admitted.

Aj tried to scream, to yell at her not to, but the first block prevented her from breathing much, and she could do nothing as the broken leg was a back one. Twilight mounted her, cock sliding into her tight, clenching pussy. "Oh AJ...that's tight...oh jeez, it hurts doesn't it? Well, alright...I dont think this will affect it much," she panted, flexing her hips as she slowly raped her friend.

She carefully levitated a needle over, injecting AJ. A soothing numbness spread, the pain abating slowly. She tried to writhe, but just tore muscles and ligiments she could no longer feel. Since that had been all keeping her broken bones in, they tore through her skin. Again, she could barely feel it, like a dull ache, but Twilight watched in fascination as she made unwanted love to AJ's pony pussy. She took notes on autopilot, eyes wide to take it all in.

Another block on the body, and ribs began to creak. Another, and they cracked. One broke enough to puncture organs, causing even more agony even through the drugs. AJ tried desperately to at least suck in another breath, but all she got was another block, this one breaking her ribs, finally squishing her.

Her guts came out of her mouth, and some out of her ass, but Twilight never stopped fucking her cunt, blowing her load during the deathgasm clenching. She pulled out, studying the blown out asshole, before she wrapped the guts around her cock and began to masturbate guiltily with them. It was just too good! Like reading a book, or finishing a checklist!

Looking at the remains of two of her best friends, she pondered who to go to next...this was their mane group, save Spike of course...and she thought he might be more interested, and useful, in helping. That, and she'd raised him...she'd rather use a different dragon if she decided to do them at all-there was enough on anatomy for them, surely.

More unicorns, of course! Repeat experiments, more stress testing in different ways? Her mentor, her sister... her breathing quickened with anxiety, guilt and lust. Could she do it? Better yet...would Celestia LET her? So little was known about alicorns! She got excited, mounting a hole in AJ's side and fucking it furiously. Oh Celestia yes, let the goddess be willing like Pinkie! Her last moments, so erotic! So romantic, just for science and Twilight! Just for-oops. She'd gone off, bloating a lung. She pulled out, starting to clean up.

She suddenly grinned, remembering that Pinkie's siblings were coming tomorrow-she could, of course, have called it all off...but how else would she get Maud there without setting off Maud Sense? Like Pinkie, a marvel in her own right...yes... A royal invite to a party...why would they question it when their sister was the royal party planner for Twilight? Big Mac liked Marble, didn't he? Maybe he'd like to show up too...

Twilight Does Science for Fun II

Limestone whimpered, trying to shuffle away on broken joints, mouth drooling blood and the odd missed tooth. Big Mac simply followed her-without legs, she was fucked. In more than one way. She tried to ignore the sounds Twilight made fucking her sister's fresh corpse, trying to get to the closed door leading to the stairs. She didn't know how she'd get up them, or what she'd do...but she had to try. She wouldn't risk dying without trying her hardest, with out fighting them to the bitter end.

It didn't matter to anything but her pride, as Mac seized her broken ankle joint in his muzzle, making her scream in fright and pain, before he twisted his head. Although it did get her on her back, it ripped her hoof free. He spat it to the side-his instructions hadn't included that, after all.

"Mac, don't! E-even if you won't do it for me, do it for you! You really think Maud will let this go?! I...I can ask for her to go easy on you! I..." she trailed off, seeing the tears wetting his face, and realized he wasn't doing any of this on purpose. He was as much a victim as she was...and no doubt he'd suffer too. She sniffled, before nodding. "I forgive you," she murmured where only he could hear. "It's not your fault...but please...make it fast if you can't make it painless,"

He seemed to nod slightly, using his front hooves to lift her rump up, before thrusting into her ass so hard it ripped. She screamed, wailing in agony. But she knew it was best-surely, the more it hurt, the closer she'd be to dying. Unlike Twilight, she had no idea how much punishment a pony body could take before giving out.

Mac shoved his cock in, deep and violent, long enough to actual begin bruising the walls of her intestines. Twilight cast an xray spell so she could watch the thick meat ravage the depths of Limestone. "Good Mac, good! As deep as you can, I want to see if you can get in her stomach!" He humped violently, trying desperately to use the magic order on him to end Limestone as fast as he could. Twilight either didn't pick up on it, or didn't care. Instead, she watched, pleasuring herself with the corpse of her victim, taking deep, slow strokes into the ruins of Marble's womb.

The corpse's belly jiggled with the fat load of Mac inside it, Twilight enjoying the wet sounds and movement. She ran her hooves over the still limp corpse, running her hooves down to smush the teats about. She moved Marble so Limestone could see the alicorn cock defiling her sister, thrusts splattering cum and corpse cunt honey over the other two, Limey sobbing bitterly now. "Stop...can't you at least not do that?! Or not in front of me! You crazy bitch!"

"I could stop...but oooh...it just feels so good!" she moaned, wrapping her hooves around the corpses hips and shoving furiously in her ecstasy. She ran her hooves over the bruised, swollen flesh. "Look at her...I can hear her body coagulating in her body! S...so...so hot! Gonna cum!" she exploded cum deep inside, adding to the bloated tummy. She pulled out with a satisfied sigh.

"Well, Limey...you want to die, right? And it won't happen until I say so. So, you should do what I say so it does happen..." she smiled, almost apologetically, but felt she should get in some data about how a pony's mind broke just as much as their body. She pushed Marble to her sister's horrified face, dripping with multiple affections and blood. "And I say...eat her out. Deep. Get mouthfuls of that cumshake, and gulp it down."

Limestone began to fight, kicking Big Mac and trying to wriggle away with her broken legs. "No! I won't! Never, you can't make me, I won't!" she wailed, sobbing in pain, terror and misery. Twilight sighed, "Then I guess we can start taking the bits needed for cunnilingus off of you... Big Mac, start with her jaw. Work it free, nice and slow."

Limestone tensed, as Big Mac aimed a stomp, and began to knock her jaw off. At least there were no teeth to hurt too much during each stomp. The flesh began to pull away from her jaw and face, her jaw dislocating with a wet, meaty pop. Mac leaned down, taking her jaw in his muzzle, and slowly twisted left and right, loosening it even more. He pulled it free, placing it down next to her as she sobbed wetly, gurgling blood. However, she pulled Marble's corpse rump close, and began at least trying to eat her out.

Twilight nodded, "Good! Show me what a good little incest snuff slut you are, and I might let your suffering end," She sobbed into the corpse cunt, lubricating it further with her tears. She did her best to swallow the nasty concoction, but had little success with her tongue flapping about. Twilight straddled Marble's messy middle, sitting on it, sending a wave of gore and cum into Limestone's face who, to her credit, tried to gulp down as much as she could. Twilight pulled her head back, letting her use Marble's cunt as a pillow.

She looked to Big Mac, "Put your hoof in her, and don't stop until you come out her muzzle," she felt bad for the once strong pony-time to begin the end of her. That didn't stop her from casting a spell to see how the damage progressed as Big Mac's hoof did.

Mac pulled out, trying his best to get his regret and sorrow across. Limestone closed her eyes as Mac lifted her bottom, pulling out, and shoved his muscular, rough hoof into her already chaffed, bloody ass hole. She whimpered, trying to hold it in. Mac thrust deep and hard, as fast as he could. Soon she was coughing up blood, followed by chunks of her internals. Twilight watched with fascination, one hoof sliding to rub at her own soaked cunt. The spell let her see the progress of Mac's hoof and the damage done. Soon she didn't need it, Limestone gagging, puking up the food she'd eaten along with all her organs. The gore and vomit fulled her lungs, drowning her. However, Twilight sneakily cast a spell, keeping her alive and breathing, even if it was agony. She felt the worst about that one, but she had plenty of data, and a cunt that was basically a faucet.

She thrashed a last time, trying to empty her lungs, but finally her body hit its death throes, Twilight timing her own orgasm so she blew a load onto her face as Limestone died. Twilight moaned, rolling off, before moving the corpse sisters aside. Instead, she pulled out AJ's broken, flat body. She released Mac too, she could tell he was far too weak to fight her even if she wasn't an overpowered ruler. He collapsed, sobbing silently, mourning Marble and Applejack.

"Mac. If you don't want me to science more of your family...you'll do as I tell you," Mac looked up at her, hate in his watering eyes, but nodded slowly. "Good boy! Fuck your sister, and cum in her." he recoiled in disgust, looking at the flat pony, then at the mad scientist. "And, no...I won't be using magic to get you hard. You have to do this on your own...or you end up scienced, as well as your family...all of them, and you know I can do it. I'll make you watch for some of them before I experiment on you too." she was curious to see how far he'd go on his own with just that threat.

He trembled with fear and rage, but managed to stumble to AJ, taking her pulverized pelvis in his front hooves as he sat on his haunches. He pulled her to his crotch before pushing her away a few times, hesitating. Twilight simply watched-she wasn't on a time crunch, and honestly, the emotional misery made her as wet and hard as the physical misery did. She rubbed her cock with one hoof, the other rubbing her sopping pussy.

Mac closed his eyes, trying his best to pretend she was warm, alive, and willing. It helped, a bit. His massive cock slowly spilled forth. He took a deep breath, running his hooves over his sister's muscular flanks, ignoring any broken bones or flesh, pretending the blood he felt was sticky cum from an earlier session. To Twilight, it was kind of boring, but she allowed it...for now. Mac rubbed his cock between AJ's ass cheeks, before he slowly pressed into her cunt, shivering from the coldness enveloping him, and began to go soft. He sobbed, knowing if he couldn't do it, this crazy bitch would kill all of his family.

Twilight pulled the corpse away with her magic, Mac reaching for it with terrified, wide eyes. "Tsk tsk...far too soft to manage any of it, Big Mac...but that's okay," she said soothingly, as her horn glowed, placing the spell back on him, and teleporting a certain grey earth pony to them, still slumbering. "Because we still have so much to do while we anticipate your family's misery and end together," Again, she wasn't sure if she could-but that wasn't the point. He'd die thinking she did no matter what, and she petted his mane as Mac could do nothing but cry.

Twilight Does Science for Fun III

The first thing Twilight did was layer Maud's body with weakness spells. She'd seen the pony smash through boulders with ease, and as curious as she was about her own, alicorn durability, she knew they wouldn't record it and that was the most important thing. She lifted one of the earth pony's hooves in her magic, studying it. She decided to cripple her as she had Limestone-she wasn't going to take the death of all her sisters well, she knew.

She twisted the legs, starting at the hooves and moving up joint by joint, watching Maud's face as it twitched in pain, making Twilight's pussy juice up enthusiastically, cock now hard and slapping her belly. She waggled her rear at Big Mac, "Mount me, I wanna be fucked while I do this!" she moaned.

Under her spell, he had no choice but to mount his sister's killer, stretching out her juicy cunt with his size. She moaned, moving her hips back onto his cunt swab, meeting him as he began to thrust. He glared at the back of her head, all he could do, as he wished he could fuck her to death right there and then.

She was oblivious, but she wouldn't care if she did know. With each snap of the joint, she clamped down on him, and he couldn't help but enjoy it, knocking at the door to her womb. She thrust herself back as he forward, ripping her cervix open violently. She screamed, and for a blissful moment he thought the sick cunt had murdered herself, but it became clear she was enjoying the pain and her alicorn healing factor ensured she wouldn't pass from just this.

"It...it hurts! But oh Celestia, I'm going to cum so hard!" She mewled, using her magic to jerk herself off furiously, almost painfully, as she abused her pussy on Mac's cock. She finally went off, soaking behind and in front of her, splattering Maud's belly with her spunk.

Twilight pulled herself off Mac, his cock throbbing in protest at the early withdrawal. Twilight pulled the pieces of Pinkie over to her, making sure the other two were in sight as well, although Pinkie was the main event. She rubbed her soaked cunt on Pinkie's detached head, making it wet with femme cum.

She pushed Maud's muzzle against Pinkie's corpse cunt, and then magically roused her from the drugged sleep. Maud stirred, mumbling, licking her lips at the taste of her favored sister. She frowned, realizing something was wrong, opening her eyes...to be met with the bodies of her sisters, mauled, raped in every way, Pinkie in literal pieces with an expression that was far more pleased than it should be.

The agony in her limbs hit her at once, making her grit her teeth, looking around in a fury she couldn't act upon. Twilight smiled at her in an unhinged way, Mac's fur wet with sweat and his own tears. Twilight petted Maud's hair, who tried to bite her. The princess chuckled, unperturbed. "Hello, Maud...welcome to my experiments! Oh I've had so much fun, and learnt so much about the body and its durability! And now...I'm going to learn about the mind!"

She pushed Maud's head back down to Pinkie's pussy. "Eat up! And I know, I know...what would I do if you refused?" she straddled the earth pony's back, thrusting her cock against her spine. "W-well, I...I have some spare nails, you see...a-and I..." she moaned, moving faster. "So much! I shouldn't, but oh, I can't help it! Learning while I get off...it's too much to resist!"

Maud shivered...she was insane, lost it. ...she'd bide her time, the longer she played along, the more likely she was to be rescued. "I will....comply," she agreed, turning her gaze to the sperm dripping pussy. ...this was going to be much harder than simply saying she would do it. She leaned in, before leaning back, and then in again, tongue lapping at the clitoris.

Twilight leaned over her head, watching. "G-good...put...put your tongue in her...french her good and deep!" she encouraged, rubbing her cock with her magic now. Maud pushed her tongue between the pink folds, before gagging, retreating before she threw up.

She took a deep breath, she had to...she needed to at least try, to survive this. She pressed her tongue into the dry, frigid folds, only Twilight's slightly fresh sperm giving the hole any warmth. Maud resisting crying-she didn't know if that was something the mad ruler wanted, but she wouldn't chance it. She did wonder briefly if that would, at least, make the dry folds easier to lick. She twitched her front hooves, wanting instinctively to turn up her favored sister's bottom to dyke out properly, pushing down the pain that came with it.

She slurped deep, pretending Pinkie was alive, just filled with party favor. It didn't work, and she turned her head to retch, but kept her lunch down at least. She realized with a sinking feeling she might not survive this...just like all of her sisters. Her eyes watered, but she blinked it back.

"I cannot. Can I do something else?" Twilight thought on this, "Hm...well, it's what I needed you to do...Big Mac! Shove your cock in her throat, don't stop until she's dead," she ordered, Mac stumbling over to Maud, weak from the constant spell and his attempts to resist it. "I would not do that-I will bite it off if I must," Maud intoned, Mac looking almost relieved that it would stop him from having to keep helping Twilight.

"Oh, could you?! He's so big and strong, I'd love to see how long it takes him to bleed out!" she enthused, as Mac's possessed body shoved his cock in Maud's mouth. She struggled despite her broken limbs, managing nothing more than a slightly humorous flopping about. Twilight thought so at least, giggling softly.

Mac held Maud's head to his groin, to the hilt in her throat. He looked at her apologetically, unable to actually vocalize his regret. She looked much the same, if so much more muted. She tensed her jaw, and he closed his eyes, not resisting in the least, although he did scream in agony as her teeth clenched, jaws used to chewing rocks easily meeting through his meat.

He pulled back, and she kept her teeth clenched, and he was grateful for it-he'd be able to bleed out and die, joining his sister. His cock tore raggedly, letting go with a tearing sound. He collapsed backwards, staring blankly at the roof as he waited. Maud spat the cock out, fixing Twilight with a blank, but somehow furious stare.

Twilight levitated over a quill and parchment, excitedly scribbling it all down as she observed, occasionally poking the comatose Mac with questions. He barely answered, but she listened raptly, excited-rather obviously, as she could no longer contain herself and mounted the hole he now had instead of a cock. She pressed in, moaning, somehow still magically scribbling notes on parchment, recording her own experiences in the moist, false cunt.

Mac winced, teeth gritting, desperately hoping she was forcing out more blood instead of blocking it and causing him to linger-which was, in fact, what was happening. Twilight turned her attention to Maud, a mad glint in her eyes. "I'm impressed, Maud...how do you stay so stoic? Even Limestone couldn't keep it up...and honestly...I don't think you'll be able to either, with the right encouragement,"

She levitated over, from a wooden storage box, a hammer and packet of nails. "It's almost too bad you didn't get to see Pinkie die-but I don't think that would have worked anyway...she enjoyed it, asked for it even!" she sighed, in loving memory, "What a true, true friend."

She paused, noticing Mac going into his death throes, and she savored the feeling of a deathgasm from the cock hole she was plowing, noting how different it felt from an asshole or cunt. She blew a massive load into him as he died, the last thing he felt was his sister's crazed killer's sperm shooting up through his body. He shuddered to a stop, and Twilight pulled out with a satisfied sigh, trotting over to Maud's infirm body.

Maud fixed her with a blank, somehow warning stare. "You are mad," she stated simply. "You should turn yourself in before it gets worse." "Oh, I could never do that! My research needs to be finished before I show Celestia, and then we can decided what Alicorn should be researched! I would offer, but well...I worry the others might not be as meticulous as they should be!"

Maud stared in response. Madness, absolute madness from this pony. She thought about Nightmare Moon, and wondered if this was some sort of weird Alicorn puberty. It seemed likely...she suppressed a shudder at what Cadance might become. Rape-crazed, no doubt.

Twilight stood on Maud-a hoof on each broken, now swollen, limb. She levitated a nail out of the pack, smiling at the single sweat drop rolling down Maud's forehead. "Oh good, you still have feeling in them!" the last of her misgivings and reluctance was being swept away in a tidal wave of ecstasy and knowledge. She pressed the nail to the soft underside of Maud's hoof, her limbs so twisted the bottom now faced up. Twilight hammered it in, Maud's body twitching involuntarily.

Maud tried to pull away, teeth gritted, but Twilight hammered it into the ground, not stopping until the head was flush against Maud's hoof flesh. She did it again, five times, imagining it was her own cutie mark star. A little...signature, if you will, on her work. Maud's face was strained with the effort of not screaming, or even crying.

Twilight noted all this down, as she repeated the star of nails on each and every hoof. By the end, Maud was gasping, eyes wide and pupils tiny. She was almost at her limit, and Twilight could tell, full of glee. She sat on Maud's back, rubbing her cock through the earth pony's mane, enjoying the feeling. She stood, rolling Maud onto her back, heedless of the nails still pinning her hooves to the floor.

The flesh and nails both began to part, making a horrid ripping sound. Maud made a gasping noise, deep in her throat, shaking violently now. Twilight ignored it aside from making a note of it, focusing on slowly turning and ripping Maud's hooves apart. Finally, Maud let out a desperate wail, bleeding out and internally, already so close to death.

Coughing up blood she wheezed, "End...it...please, no....more..." Twilight stroked her mane, gently. "Alright," she agreed "Time to end this experiment," But to Maud's horror, she levitated over the mangled remains of Pinkie's rear. "I...I still cannot-" "Oh you won't be, don't worry!" she assured her.

Twilight made sure Pinkie's pussy was nice and snug as she pushed it over Maud's muzzle, preventing her from taking anything approximating a breath. Twilight kept her muzzle shut with magic, the deceased pussy sliding over the muzzle easily, until Maud was up to her eyes in her sisters cunt. She struggled to bring in a breath, any little air that might have been in the corpse cunt quickly used up.

Even then Twilight didn't stop, forcing Pinkie's well used puss over her sister's eyes, shoving until Maud's head was in her tunnel, and then with one final heave, it popped right into Pinkie's womb. Slick with cum, it at least slid over the pony head easier than Twilight thought, and it made her wet and hard to imagine Maud trying to draw in breath and drowning in the alicorns cum deposits.

At first she tried to struggle, her broken hooves rubbing ineffectively against the dry, dead flesh, before letting out a broken, pained sob, and going limp, only her lungs trying to instinctively fight, to draw breath and live. Twilight pressed into Maud's pussy, thrusting calmly, enjoying the feeling of dying, raped cunt-it was rather relaxing to her now.

Maud's pussy spasmed randomly around her as her body fought, massaging the invading rape stick nicely. Twilight slid her hooves over the pony's chest, feeling the heart beat as it fought to live, taking yet more notes on it all. Maud's heart became erratic, her body giving one last, panicked thrashing in an attempt to dislodge the corpse around its face and live.

Twilight reveled in the deathgasm, thrusting as she enjoyed every moment of Maud's death, the earth pony's pussy practically sucking the seed from the alicorn's magicock. She obliged the hungry cunt, blowing a massive load of sperm as Maud slipped off the mortal coil, feeling rather proud to be fucking a science corpse of her own making. She kept going, her magicock staying hard, in order to blow a second load. She chose to pull out of the pussy, pressing into the tight tailhole and unleashing a flurry of thrusts, the asshole tearing at her rapid, uncaring and uncareful thrusts inside. The corpse was just fresh enough to still be oozing blood at this abuse, and it got Twilight off even harder.

She moaned, filling up the bowels as she had the womb, before yanking free with a satisfied sigh. She cast a spell on all of the bodies to keep them fresh, wanting to enjoy and display her experiments for a long time to come. She floated over all of her notes, settling down at a table to begin writing her book in earnest-soon, she'd be able to unveil it to Princess Celestia herself!

Twilight Shares Her Love of Science with Her Mentor

Twilight danced in place eagerly, her magic collecting all of her comprised journals and original notes too. Her friends, whether pieces or whole, were in special displays with short descriptions of the experiment performed. Finally, she had all of it collected, and Princess Celestia waited for her-with Luna too! The princesses she admired were to be the FIRST to see her science! To see how she'd furthered pony science for generations to come.

Proudly, she cast a massive teleport spell, landing in the hall-empty of even servants, as she'd asked-and began setting up. A long table for the reading and discussion to come. She double checked her work, her careful penmanship and detailed drawings in her final journals, and the preservation spells on those in the displays. She paused, before adding a protection spell to it all. Just her luck if something was shattered, or ripped, or Celestia forbid! Tea got spilled. Accidents happened, but she would be prepared for it all, she thought, nodding decisively and proudly.

She sat at the head of the table, just as the royal sisters entered, Luna looking a little tired. "Ah, Twilight! Goodness, this seems very thorough," Celestia complimented, Luna looking a bit more disturbed. "It is...very lifelike...?"

"You don't know for sure, do you Princess? It's okay, neither did I for the longest time. How empty are our medical textbooks! We rely on unicorn magic far too much, and I fear we may have lost some unneeded," she said, mournfully, before cheering up. "Which is why my friends helped me with this!"

Celestia sat down to read, as Luna perused the displays, looking a little sick. After a short while, she stumbled back, neighing in distress. "Sister! These...I think these are real!"

"Well of course," came her sister's gentle voice. "How else would Twilight be able to study this all properly?" Luna seemed to pale under her dark coat.

Celestia looked up, "I know. However...think on the thousands these lives will save. I mourn their lose, as I praise their selflessness. Some of these experiments do seem...extreme...but better a few suffer and die than many. Such is the weight of our crown, sister."

Luna sat down slowly. It made sense, admittedly, but it still disgusted her. Twilight smiled reassuringly at her, "You don't have to stay, Princess Luna. I know it can be hard to look at first, and it's better to work up to it than it is to force it." It was these words that seemed to calm Luna. Perhaps the victims-subjects-really had agreed, and this was an unfortunate misunderstanding. A mad mare, bent on harm, would not show her plans to her victims like this.

Celestia nodded, putting the last journal down. "I am impressed, Twilight...you've been thorough, added detailed cross sections when needed, and preserved your friends so wonderfully. However..."

Twilight paled, nervousness on her face. "Yes, princess?"

"I see no alicorns here."

"Oh!" Twilight said in relief, laughing. "Sorry, it's just...I was just as disappointed. I considered having someone do me, but well...I had no guarantee they'd do it in the depth I aim for. The detail, each minor thing recorded just in case."

Celestia nodded, "As I expected from my prize pupil! Now...as we will be retiring soon...I wish to offer myself as a subject."

"Sister, hast thou gone mad?!"

"Now now Luna...with alicorns being born into this world, knowledge about ourselves even we lack is needed. I know I am giving up much, but if my decision helps even one of my little ponies in my wake, then I consider it a good one."

Luna hung her head. It made a twisted sort of sense, and she agreed only Twilight could truly do it justice. She gave it more thought. Being alone again...she did not look forward to it. Well...here was a way out, that would benefit their little ponies, and ensure she would not be condemned for suicide.

"You are right, sister...and when she is done with you, I would like to offer myself for a better study. One alicorn cannot show what is usual for us and what is unique to you, sister." Luna reasoned, Celestia nodding.
"A fair point," she conceded. "But my turn is first! Shall we do it here, Twilight, since you brought your tools with you?"

"Oh, absolutely!" she enthused, wings erect. "One moment..." she carefully cleared the table of her work, putting it aside where it wouldn't be damaged, and summoned a very large, emtpy journal. She thought she might need it to fill what new discoveries Celestia would help her uncover.

Celestia figure out what she might want, jumping up onto the table. "Shall I lay down, Twilight?"

Twilight shook her head. "No, princess-stay still, wings out please. I want to start with them once I've finished recording things," she explained, measuring the princess in many ways with a multitude of science tools, from a tape measure to calipers. She pulled a feather out, not eliciting so much as a wince-she recorded that too.

Celestia did flinch a bit when she felt her royal lower lips gently held apart by magic. "Oh, sorry Princess! I should have warned you,"

"It is fine Twilight, I knew you were thorough and should have expected it," the sun princess giggled, abashed at her own thoughtlessness.

"You have a hymen, princess-are you, um..."

"Yes and no. There is no reason to hide it further-my little ponies see me as a...pure being, a goddess, so I have to keep up appearances. I am still a mare, however, so I reconstruct my purity after each session that breaks it."

"This is good, princess! I can get at least some data with this, how durable this part is." she enthused, the sun princess chuckling happily at her enthusiasm.

"Okay...I won't be using any anesthetic, so please stay as still as possible for as long as possible," she warned, Celestia nodding.

A scalpel was levitated to her back, following lines only Twilight could visualize, cutting her wings carefully, flaying the skin open. Twilight gently plucked each and every ivory feather, collecting them in a sealed bag. Celestia stayed still, her breathing increased due to the pain. Twilight recorded that too, pausing to ask questions and Celestia being very helpful and in depth with her answers.

Celestia's white coat was soon stained red and pink, and Twilight had her move back, resting her chest on the table, back legs on the floor. Twilight rubbed one of Celestia's cutie marks, enjoying how thick she felt. She cast a spell on herself, giving herself a thick, fat horse cock. She mounted up on the rump, to Celestia's slight surprise. However, she wrapped her tail warmly around her student's body.

She turned her head enough so Twilight, leaning forward, could kiss her. They made out as Twilight rubbed her magicock against Celestia's almost uncomfortably hot holes, warm cunt juice dripping down the princess' normally pristine, perfect coat. Twilight made sure to get absolutely drenched in her mentor's cunt sap, only magic holding back a monster orgasm already.

She pressed the cock tip to the remade virginity, pressing against it testingly. Celestia moaned, the feeling of a hot cock getting ready to plow her depths made her drip more. Luna watched in curiosity, a faint blush betraying what kind of curiosity.

As Twilight pressed into the depths, she leaned forward to nibble on Celestia's ear lovingly, the princess leaning back into the younger's ministrations. Both let out cries of ecstasy as Twilight's cock thrust through Celestia's virginity. Luna moved to watch from the floor, close enough to feel Twilight's tail brushing her face. She hardly minded, having an excellent view to her sister's fucking. The thick, purple cock, spreading her sister's petals, the pretty soft pink of her inner tunnel revealed to the world. Each thrust opened the flower, it's nectar falling like summer rain upon the moon mare.

Luna licked her face, tasting her sister's excitement. She did question how she could enjoy being butchered and fucked simultaneously, but couldn't deny it was...hot in a very twisted sort of way. She slid a shoe off, reaching back to tend to her own needy garden.

Twilight slid in and out of the heat slowly, relishing each and every inch and moment. The godly depths were hot, gripping her cock with centuries of experience. The muscles practically suckled her, begging for her impotent seed, demanding a fill. She hooked her forelegs over the larger's flanks, and hammered away desperately.

"Oh, Celestia! I...I'm going to cum! My first load!" she promised, before crying out as her cock shot a truly prodigious amount of seed into the flaming cooch. Celestia moaned, shivering, not cumming herself yet but oh so close. Twilight nuzzled her mane, before moving just enough to stand with her forelegs keeping her balance on Celestia's suns.

Twilight brought over a scalpel, carefully cutting away the wings and detaching them at the joints. She sighed, her cock hardening again inside of Celestia's randomly twitching and clutching cunt, the pain making her tense. Twilight petted her mane. "I'm going to skin you while I fuck you and record it,"

Celestia nodded. "I understand. I will try not to writhe too badly-an intact alicorn hide will be something to marvel at," she admitted.

Twilight mounted her again, slow and steady fucking, her scalpel tracing lines along the alicorn's coat, planning where to cut to pull off the hide in an easy way. Twilight's magic took hold of Celestia's skin above her hooves, carefully loosening it with her array of surgical tools. She peeled the skin back off the muscle, revealing the twitching redness to the air. Twilight continued to ask questions, and Celestia, like a good sport, answered them.

Celestia began to shiver from the shock, even Twilight's plowing cock couldn't overshadow the agony. Blood soaked the table, the floor, all three alicorns. Twilight peeled the skin to Celestia's chest, careful still-she got to the face, removing even the eyelids intact. Each cut was made with her thrust, so as not to fumble or worse, cut the skin and ruin the hide.

Celestia's flayed upper half laid on the table, twitching randomly. Her eyes stared blankly, heart fluttering, no longer able to answer Twilight, who peeled the remaining skin free. She had to pull out of the tight cunt to take the pussy lips with the intact skin, but dived back in as soon as the skin was carefully put aside for later.

She humped furiously, panting, tongue hanging out of her mouth. She could feel Celestia's deathgasm approaching, the princess trembling and twitching on her cock. Finally, she gave one last, monumental shiver, her pussy milking Twilight's cock as her final act.

Twilight shoved it in to the base, holding herself still, letting the deathgasm milk her cock on its own. She sprayed a tsunami of maremilk into the former princess, and collapsed onto the flayed back, panting. She jumped, turning to see Luna tonguing not only Twilight's sopping pussy, but where she connected with Celestia's corpse as well.

"Well, Luna...you're next,"

Twilight Makes Sure Luna Isn't Left Out

Luna waited patiently off to the side, her sister preserved for later, proper preservation into a science attraction next to her. Twilight had moved the table to the side as well, setting up a large, collapsible pool. She started slowly, carefully, filling it with liquid. She wore gloves over her front hooves, and had on a gas mask.

Luna watched with curiosity, Twilight erecting a bubble around the pool. Luna wasn't sure what it was for, she could see and hear her work quite well still. Soon it was full almost to the very top, and Twilight teleported in what looked like a small crane and harness-Luna assumed it was most likely for her. It was too big for a normal pony and only just too small for Celestia. Twilight fussed with it, unbuckling the straps. She pushed the crane over to Luna, aided by wheels on the base.

"Alright, Princess Luna, this is your experiment-I'm not only going to take notes, but pictures of the various stages you'll go through. This is acid of my own make-it'll eat through hair, flesh, and muscle, but leave your bones clean and polished for display." Twilight explained, as Luna walked into the harness, helping Twilight buckle her in with her magic.

The harness supported her chest with holes for her fore limbs, a buckle under each wing keeping them spread out as it hurt to fold them in, and another support with leg holes around her nethers. Her head was unrestrained, as were her legs. Twilight had the crane lift her up, and her legs dangled under her freely. It was an odd feeling, being moved over to the pool in the air while not flying.

Luna soon realised what the bubble was for-the smell of the acid was strong, and made her cough and her eyes water. Twilight enchanted a camera to float and take pictures randomly and when she instructed it as well. It snapped a shot of her from below, showing she wasn't yet in the acid and what she looked like before.

Twilight sat down, her magicock still stiff between her legs, one hoof rubbing it while the other tapped commands onto the crane's input. With a slight lurch, Luna began to descend-slowly. Very slowly. This was going to be painful, she mused, but then again...if it helped both their little ponies understand more of their own and an alicorns anatomy, helping the next generation, then it would be worth it. Hopefully.

Her hooves, unshod for the first time in so long, just barely dipping in the acid. She jolted, yanking them up again-she hadn't expected that to hurt so much, not until it hit above the hooves at least. Twilight watched her with great interest, and Luna let out a long, calming breath, gently extending her hooves until they hit the surface, and it became much harder to hold on to her decorum.

It burned and hissed on her, stripping the outer layer of her hoof off, leaving just the hoof and bone. It worked fast, no wonder Twilight wanted her to be lowered in slowly. Luna struggled despite herself, splashing the acid about, getting flecks on her coat and face, burning holes in herself.

Twilight watched carefully, noting it all down, including when Luna began to neigh in distress. As she was lowered further, she splashed more fiercely until her legs went limp, now too damaged to move more than a tiny sway. As flesh and muscle was burnt away, her bones were cleaned and fell to the bottom of the tank with soft tinkling noises.

Her eyes burnt from the fumes, her vision burring as they were damaged by it. Her wings ruffled, but she didn't dare try to flap them-she'd be coated in agony, although she considered it as the tiny flecks from earlier ate hungrily into her flesh, expanding to be as wide as a bit and twice as deep. Her blood seeped out of the wounds, diluting the acid, slowing the eating and dragging out her demise further.

Soon Luna was held at a depth that would have had her hooves on the bottom if she still had them, only her neck and head above the acid. The camera took snapshots all the while, a panting Twilight with her mask still on moving to stand on top of the crane's command. Luna was confused until she saw Twilight furiously jacking off to all of this.

Luna looked away, gore and blood surrounding her as she was held up and floated partially. Twilight moaned and her money shot hit Luna right in the face. Luna shook her face in surprise, as Twilight grinned and used her hind leg to flip down the crane's lever, and Luna was dunked in the acid. She screamed, doing nothing but swallowing acid and gore.

The lever was cranked up and she was brought up just enough to break the surface with her head. Her eyes were melting down her face out of her skull, her tongue in ragged slices and falling out of her maw, each tooth coming loose and slowly plopping into the acid. Like her bones, they hit the bottom with a rattle.

She thrashed, the thin skin on her belly finally rupturing, spilling her guts out into the acid, some of them already spotted with holes from her swallowing the acid. Her intestines slopped out first, dragging her stomach with it. She gasped with agony as she felt the acid burn at her heart, an impossible agony. She let out a final, terrified, pained whimper, and her head sunk beneath the acid.

Twilight went over her notes as she waited for Luna to dissolve. It had gone well, the camera had taken pictures as needed and told, and she couldn't wait to publish her findings! Of course, there was a final adult alicorn to experiment on first... Summoning a scroll, she wrote a letter, and sent it to the Crystal Empire. It was time for a visit to her favorite sister-in-law.

Twilight Bonds With Family

The train pulled into the Crystal Empire station, seeing a number of ponies disembark, including a happily trotting Twilight Sparkle, levitating a suitcase full of science next to her. She headed into the Empire proper, right for the castle.

Cadance greeted her, the pair doing their ladybug dance, Twilight even more giddy than usual. They embraced, "Oh Twilight! We missed you!" Cadance sighed, the mares trotting into the castle and into the ruler's private wing.

"Your brother's been going on and on since we got your letter- and i cant blame him!" she laughed.

Twilight nodded, "I'm glad you're both so happy to have read it, I was a little...anxious," she admitted.

"Well I think it's a great idea...and well, this'll help both you and Flurry Heart now, and whatever alicorns come after you."

"So, you already..."

"Yes. Shining wants to raise Flurry first, so it's just me today." Cadance grinned.

Twilight smiled, "Well that's good. And I...might already have ideas for you." she admitted, as they entered the Crystal Castle. "Which is what this suitcase is for-it has so much in it!"

"So, Twily...I was thinking. I kind of have a last request, I guess?"

"Oh of course! Anything, Cadance. You know that."

"Good! I want Shining to be fucking you while we do this." Cadance announced, Twilight fumbling her suitcase, stammering. "What? You've done all this, but you don't wanna get some biology going?" she teased the smaller alicorn.

This gave Twilight pause. She had done a lot...so many things, all in the name of science...so why not this? After all, Shiny had proven he could father alicorns, and that could be useful... "M-maybe," she stuttered, giving it a good think.

The pair trotted into the hallways, heading right for the royal couple's bedroom. Twilight took that time to muse over everything she'd done, and planned to do. Planned to do...that could wait. A nice break, making many little alicorns with her brother. She blushed at that, then shook her head. Focus! Science time!

They arrived at the bedroom, Cadance trotting in excitedly, followed by a blushing Twilight. Shining was waiting for them, wearing nothing but his fur. He grinned at Twilight in a way that let her know he was in on it, too. She shot back a matching smirk, and turned to Cadance, putting her suitcase down.

"Alright. Mostly I've tried stress tests on bodies externally...now I want to see what happens when too much stress is placed on internals. But you're my sister in law, so I wanted to do something you'd enjoy too and well...it suits you being the Alicorn of Love. Basically...I'm going to use both magic and science to induce orgasms beyond what your body should allow, until your heart gives out," Twilight explained, Cadance doing an excited little dance.

"That sounds amazing! How do you want me for this?" she grinned, snoot to snoot with Twilight.

"On the bed-I'll need to tie you down so you don't spoil the experiment." she explained. Shining chuckled, floating out bdsm gear from a dresser.

"Like this, Twily?"

"Oh! Uh, um, yes...can you truss her up then while I get the gear ready? Oh! And she'll need a bridle with a bit so she doesn't bite her tongue." she explained, Shining nodding.

He trotted over to his wife, and began. He started with the bridle and bit, Cadance sticking her tongue out to tease him, giving him bedroom eyes. He smirked, running a hand down her neck and to her chest, taking her peytral and moving to her fore legs, placing the in a spreader brace. With a playful nibble at her chest fluff, he moved to her wings, gently folding them to her sides as they'd sprung up in excitement. He belted them to her sides gently, not wanting her to chafe or hurt. He moved to her hind legs, nipping at her cutie mark in a way that made her tail lift. Her back legs were restrained as her front ones were, with another pole between them to hold her steady. He grabbed her tail last, tying it to a ring on the back of her bridle, keeping her head and tail up.

Twilight nodded, a number of items floating in her magic. "That's perfect, Shiny! Okay, now, Cadance...this first one goes in the vagina. It's got a plug at the front to hold it in your uterus, so you MAY feel a pinch as it goes in. The second one goes in the anus, and that plug's very wide. I'll be as gentle as possible, but if you still tear, don't worry! I'll remember to note it down." Cadance could only moan in response, even as she tried to wiggle her bottom to encourage her.

The first one was pressed into her warm, wet folds. It was very large- her labia spread around it, stretching her out in a way only pregnancy had managed before. She whimpered, wiggling very slightly. Twilight's tail was up, her legs spread wide, enjoying both what she was doing and how it hurt Cadance. Twilight thrust the first device back and forth, Cadance bleeding slightly. She noted this down, before finally pressing it deep within, and once she felt the cervix, pulled back before slamming it in and continuing to press it in. It went in with a pop, making Cadance scream around the bit, and Shining mount Twilight.

Shining's cock pressed against Twilight's cunt, his flat head large and demanding, before he simply thrust it in. "OW! Big brother, it hurts!" she complained, almost dropping the things in her magic.

"Well of course it is. You need some data on yourself, right? Now get back to multitasking," he instructed, taking long, hard strokes to her own cervix and back. She whimpered, it hurt...but it set her afire at the same time. She nodded, and began pressing the massive buttplug into Cadance, who screamed and tried to thrash away. Twilight began thrusting this one too, matching the thrusts from her brother, each thrust getting a new muffled scream.

She finally jammed it in harder, Cadance's asshole tearing as it lodged in deeply. Twilight moved bullet vibrators to Cadance's teats, using a spell to be able to press them inside her nipples, clamps keeping them in. With another spell, all four of them began to pulse and shake, pleasuring Cadance as the magics already in them would keep her cumming.

Twilight took notes as her brother kept using her, fucking her like a fleshlight. Twilight had to admit it was getting her off. She moved her hips back onto his cock, mewling, as Cadance joined her in moaning. The vibrators in her teats were amazing, the ones in her ass and pussy stretching her out and filling her so much, each inch they touched pleasured. She gasped, cumming, hips shaking. Twilight whined, about to join her, before her brother shoved his fat cock right up her asshole, ravaging that even harder than her pussy. She began to cry, it hurt- but the fact she now knew how it felt for some of her science partners turned her on much more.

As such, she did manage to cum, screaming in pleasured pain, her pussy gushing fluid against Shining's legs. She kept moving back onto his huge cock, her own asshole bleeding down her legs. Shining couldn't keep it in, watching his wife have another orgasm, as he fucked his sister, border lined raped, made him need to cum so very hard. With three good, hard shoves, he came right in her asshole, and pulled out to spray the last down her back. He staggered back, sitting on his haunches to catch his breath, Twilight doing the same as she wrote notes on both how Cadance was holding up, and on being fucked so roughly by her brother.

Cadance had hit the sweet spot where she was simply coming constantly, aided by the magic in the vibes. Twilight crawled close enough to put her hoof on Cadance's chest, taking her heartbeat. It was racing, Twilight grinning. She cast a spell, her magic aura now directly above Cadance's heart and attached to Twilight's hoof so she could keep an eye on it. She turned to Shining, "Alright!" she chirped, "Your turn!"

Before he could ask for what, she'd grabbed him in her magic, said magic clamping down on his own so he couldn't fight back. She cast another spell, giving herself a massive, long horsecock. "Open up that mouth, big brother! If you don't, I'll have to make you...and I really wanna make you," she panted.

He opened his muzzle reluctantly, not as fond as being on the receiving end. Still, he didn't want his sister to decide he could be an experiment anyway. She took the invitation, and holding his head steady with her hooves, shoved her cock down his throat to the base on the first thrust. Hooking her forelegs behind his head, she raped his throat with reckless abandon, making him gag and cough. Al it did was make her hornier and massage her length. She pulled out just to cumshot in his eye, pressing her cumhole right on his bare eyeball to do so.

He screamed in pain, trying to thrash away. "Oh big brother! Keep that up, and I'll have to fuck your eye out of your skull, you scream so sexy!"

He gritted his teeth, trying to keep silent. She finally puled away, leaving him to try to blink spunk out of his eye. She moved Cadance so she was at his rear, to the side, getting a good view of his balls and asshole. Twilight seized his hind legs in her magic, spreading them wide. She pressed her muzzle to his asshole, lapping at his ponut, sliding her tongue in deep.

Cadance did her best to watch, as her vision was going shaky and her heart was racing at a painful pace even as waves of pleasure rushed over her. She watched as Twilight mounted her brother, thick magicock at his entrance. Cadance had always wanted to see his asshole ruined from a big fat cock, and Twilight being the one to do it got her off harder than before. She trembled, clinging desperately to life just so the last thing she saw was Twilight getting off in his asshole.

Twilight arched her back, slamming her cock into the hilt, making her brother scream. "Nnngh, fuck yes, scream for me! I love the sound..." she reached forward to pet his mane, "It's alright, I'll make up for this...you and I are gonna be making alicorns for years! Together, with others, and when they're old enough...more science!" That did seem to calm him, enough to relax and start enjoying the buttfucking.

Twilight took long, deep strokes, tapping at the bend of his intestines with each one. "Oh this is amazing! I should do an entire series of science on incest!" Cadance had to agree, even as her breathing became more labored. Twilight took notice, "Big brother, look! Your wife's heart is collapsing from too much pleasure! Isn't that so hot?!"

Shining had to agree, the look of ultimate pained pleasure on his slutwife's face was amazing, almost angelic. Twilight redoubled her thrusting, ass bouncing, Shining rolling his hips back to take it all. They watched as Cadance trembled, eyes rolling back and teeth clenching on the bit in her mouth. Her bound wings tried to flap, struggling in their bonds, before she went stiff, and then limp in her restraints. Twilight cried out in delight, "She died! She died from cumming so much and -" she whinnied, cumming herself deep in her older brothers asshole as he shot a load onto the floor.

Shining looked over to grin at her. "So...alicorns and incest huh?" She grinned back. "Maybe..."

6 Twilight Does Science for Fun I

Twilight sent invites to Big mac, and all three of Pinkies visiting sisters. She had her special science room cleaned and prepped, taking no chances, especially when it came to Maud. She might be the most powerful non alicorn in the land...maybe more powerful physically than any alicorn. Which is why the lab was stocked with potions, spells, and horn repair items...just in case.

She went through her notes, reading up on her science, one hoof gently rubbing at her purple princess pussy as she reminisced, waiting for her guests. She knew who she'd start with, working her way up... with an excited if worried smile, she licked her hoof clean, gave Spike the day off, and hurried to the front door, timing so she got there at the first, heavy knock. Ah, Big Mac! Punctual stallion.

"Hiya Mac! Refreshments just in that room there, go have some while I greet other guests!" he nodded, "Eeyup," and trotted off to the room, a table laden with snacks and bubbly drinks that hid their spiked nature. Twilight left the door open, as the Pie sisters arrived. "Welcome! Pinkie had to run to the store, but come have a drink while we wait!"

Maud peered at her, "Pinkie. Forgot something for a party." "Ah, well, no...it's...embarrassing but um, she went to get me some...private time things," she flushed, the excuse accepted-a party pony like Pinkie had all sorts of 'private time' things, and less shame to buy them for others.

Limestone strutted in, "Well, don't expect me to be on the bottom, if it's THAT kind of Pinkie Party!" "Heheheh, it's not-but I'll remember," she assured her now dead friend's sister. She led them to the party room, Big Mac nodding to them, and blushing when he saw Marble, who returned it.

They sat at their places, each with a place card. This was needed-Maud's spiked drink was much stronger than even Big Mac's, no chances taken in this. Twilight picked up her glass. "Let's have a quick toast, to having, and making, great friends!" Everyone toasted, and drank all their juice when Twilight sculled hers, even Limestone not wanting to offend both a princess and one of her sister's best friends. As the drug began to work, they chatted and snacked, until the Pie sisters and Mac began to yawn.

"Ah, it must have been a long trip, huh girls? And Mac, you must be so tired, taking on AJ's chores as well while she's on a map mission! Tell you what, i have lots of rooms-you can all take a nap, and girls, I'll let you know when Pinkie comes back," she promised, no one seeing anything wrong with the Friendship Princess' hospitality. She led the Pies to a room with a large bed. "I thought you'd all be more comfortable together," she smiled, shifting from hoof to hoof, no one questioning a princess well know for anxiety for being somewhat anxious at a gathering.

They seemed to agree, plodding tiredly to the bed, Limestone first and laying on her side, then Marble laying on her tummy, and finally Maud laying on her back. She took the time to take Boulder and place him on the nightstand before falling asleep. Twilight nodded, levitating a potion to Maud, carefully tipping it so she swallowed it slowly, emptying it entirely.

Big Mac swayed in the hall, paying no attention to his host, until she trotted up to him. "Alright Mac!" she cast a spell, in fact multiple ones. To make him alert, unbearably horny, and completely loyal to her and willing to do anything she asked. That had been the hard part-making it look like she just HAPPENED to come across those spells, not looking for them specifically. "Science time!" she cast a spell, teleporting her, Marble, and Mac to the sciencing room.

She cast a spell to nullify the potion and wake up the almost mute pony. Marble woke up slowly, looking around, confused but not alarmed at the clean, sterile room...well, clean and sterile for now. She might be more upset if she knew Twilight's next experiment.

"Hi Marble! Now, I know you must be confused!" Twilight said, leaning down so they were muzzle to muzzle. "That's okay! See, I'm doing experiments on ponies to fill out books on anatomy! They're hard to find and get, especially in depth ones! Speaking of in depth, Big Mac here is assisting me! We're going to stress test your insides!" she explained, Marble's blush at Mac turning to confusion again.

Mac lumbered over, a bit stiffly under Twilight's control, and flipped Marble onto her back, getting between her hind legs. Marble's eyes widened in fear as she realized what the princess meant, Mac's prodigious erection spilling up her soft belly. She shook her head, whimpering, pleading with her eyes. Twilight did look a bit reluctant, but she'd already gone too far. She'd finish it, if it was the last thing she did...and it might be. She magically gifted herself with a shaft, working it with her hooves and dragging over a round burlap sack.

Marble sobbed, trying to wriggle out from the stallion, failing as he pulled her back, tip pressing against her hot little pussy. Twilight spoke, "If what Pinkie says when wasted, she won't have a hymen for you to pop-she liked to throw dildo parties with her sisters. A shame they didn't have a brother-incest would be an interesting experiment, but..."

She up ended the bag, Pinkies head and then AJ's bouncing slightly on the floor. "I kind of took care of both of your sisters already. But that's okay! If I really want incest, I have a brother myself!" Marble wailed at Pinkie's head, reaching for it. Twilight gave it to her, Marble sobbing and cradling it. "See? I'm no monster!" she said, trying to convince herself as much as Marble.

Marble screamed as Big Mac thrust, so big and long he was striking her cervix in the first thrust. Marble reached a hoof to Twilight, "Don't...have to..." "Oh I know! I've got so much data on earth ponies, this is really just for fun! Know what would be more fun?" Marble sobbed, sure she knew where this was going.

Sure enough, Twilight teleported Limestone into the room, waking her up. She saw Marble, and screamed in rage, rushing Big Mac who just kicked her away with a hoof. Her nose broke, and she slid along the floor on her side, groaning at the pain thumping through her face. Twilight sighed, before tugging Pinkie's head to Limestone, who screamed again, pouncing for it, Twilight pulling it away so she ran face first into a wall in her rage.

She staggered, landing on her belly, Twilight moving AJ away before approaching her. Her magicock was as hard as her pussy was wet, the guilty churning in her stomach seemingly only making her more aroused.

Limestone snarled, "That nasty thing comes near me, I bite it off!" "Hm, that would be an issue! I can fix it," Twilight assured her, her magic forcing open the rock pony's muzzle and practically scooping out her teeth, one by one. Limestone struggled, glaring, but refusing to sob, barely even tearing up because of the pain. Twilight thought it had barely hurt since Limestone had barely reacted, feeling good about that, at least. Until Limestone's front hoof lashed out at her, only the earth pony's dizziness from the wall hit saving the alicorn.

Twilight sighed, before her magic crushed the joints of Limestone's legs, front and back. "I know how strong Pie legs are...I won't risk it with you," she said, stroking her hair as Limestone gasped, trying to work through the pain...it wasn't easy, so much all at once. "I know it hurts...but I need the data on that. It's okay, be as loud as you need-the lab is sound proof."

Marble wailed, reaching for Limestone, sobbing. Twilight came over instead, Marble tucking her legs in again, whimpering, not wanting whatever much worse fate the princess could think up. She'd prefer the rape, no matter how physically and mentally painful it was. Twilight took Pinkies' head, lowering it to Marble. "And you? You'll kiss her, won't you? Don't make her feel unloved like your sister did, I'd have to punish you..." Twilight wasn't sure if she could hurt someone to punish them, but Marble didn't know that.

Marble whimpered, "Mh hm..." and leaned up, smooching her sister's dead face, trying not to hurl or flinch at the cold flesh pressed to her. "Don't worry! I preserved her, she'll be pretty for years, I promise," that just made Marble sob in emotional pain. She kissed deeply, terrified of this crazed pony, and hoping her exit from life would be fast, if it couldn't be painless. Twilight smiled, "Time to begin our experiment..."

She sent a bolt of magic at Big Mac, making him spasm, before he whinnied, cumming in Marble. "That's gonna make him cum a lot of times! I want to see how much it takes to pop you from the inside..." she dragged Limestone over in her magic, sitting on the earth pony's muzzle. "Lick me out, please. I know you're not much of a bottom, but I've used my cock so many times already!" Limestone glared, before lapping deep in the murderous snatch, rubbing muzzle about the silky labia. She didn't want to admit it, but princess pussy was pretty fucking good.

"Ooooh, you are good at this..." she rubbed harder against the mare's face, before creaming on it, panting, "Oh jeez...sorry about the hair trigger, science gets me going," she admitted, lifting her rump so Limestone could sputter and gasp for breath. She turned, grabbing and jerking off with Pinkie's head, before cumming through it onto Limestone's face. She gritted her teeth, trying hard not to cry at the blatant disrespect of her sisters corpse.

"M-maud won't stand for this!" "Maud is drugged harder than all of you...she won't wake up until I wake her. And we're too far down for her to hear you-I made sure of it, then went deeper, just in case." she yanked the head off her cock, letting it bounce onto Limestone's tummy. "But I'll warm you up for Mac,"

"I'm a lesbian! I'm not bi!" "Oh, sweetie...you're a science toy now. That he's going to help me experiment on, to learn more about the body...and fun, of course! It doesn't matter who the cock is attached to, once you're a corpse cock cushion!" she began to argue again, trying make Limestone see the same sense she did, as Marble screamed, body bloating.

"Ah! He must have big loads, almost time!" she said, moving between Limestone's legs and slipping in her dry, chaffing pussy. She pulled out with a cluck of her tongue, before using her own sperm to lube up and slid back in. "Wah-no! I don't want a foal!" "Doesn't matter if you're dead, although keeping you as a fuck pillow does sound tempting...I've always wondered if Cadance and Flurry are outliers, or the rule...maybe another." she said, now pumping in and out of the grunting earth pony, watching Mac unload cum every few minutes.

Marble had her hands on her tum, like she thought she could hold it all in as her womb expanded painfully. Soon, fatally. Twilight licked her lips, moving deeper. She adjusted, before ripping through the earth pony's cervix to her womb, raping her from start to end with deep moans.

Mac wrapped his hooves around Marbles hips, and shoved, the last jet of cum and the thrusting causing her skin to rip, making her wail as cum and blood oozed from the rips and tears. "Ngn...thought it would be more of an explosion...see...this is why...we need better science!" Twi gasped, cumming in Limestone's womb. The broken pony just cried silently, staring at her dying sister, knowing another was dead, she was next, and even Maud couldn't fight this crazy bitch.

Twilight pulled out, getting Mac to do the same, which made the cumming stop. She walked to the sobbing Marble, and gave her Pinkie's head. "Here, some comfort..." she said, marble taking it and making out with the corpse head. She was racked with pain, doing the only thing that could bring some comfort. Twilight let her die in peace, owing it to her best friend to let this one have it. Marble breathed her last while tonguing Pinkie's limp one, eyes going blank. Twilight nodded, satisfied, before turning the fresh corpse on its stomach and mounting up, violently raping her asshole.

"Fuck, this is good...too bad I couldn't feel her deathgasm....as Pinkies sisters, you're all very special beyond just the science!" she grinned. "Speaking of...Big mac. Take Limestone up the ass, as violent as you can...let's see how much damage she can take," she looked apologetic, "If it hurts too much, I can numb you," unaware that Limestone could never, too prideful to ask that of anyone, let alone her sisters' crazed killer.

Limestone gritted her teeth, and held down her bile. It was going to be a rough night.