Author's Note

This is for the human version of her. For Princess Twilight in human form, see this story.

Also, her scenarios might be more stretchy than the others until I can find something better, but it was the best I could think of. Also, some of these are scenes from Legend of Everfree, where nothing even slightly implies a wetting (even compared to the Rainbow Dash thing), and really she probably didn't, but, in Rick and Morty there was an episode where Summer wet her pants and no wetness was shown, so wettings can be easier to headcanon than you think, with wetness simply hidden to the audience.

Twilight Sparkle

Twilight Sparkle was very excited to be on her first sleepover with her new friends at Pinkie's house. The girls were all in their pajamas, having all kinds of slumber party fun the likes of which she had never known before. This friendship thing was really fun.

Eventually, the girls decided they all had their fun, so they climbed into their beds and went to sleep.

Twilight was dreaming that she was continuing the sleepover with her friends. They were doing all the fun things they had done when she was awake. Playing some fun games, watching movies while eating popcorn and drinking cider, pillow fights... She loved it.

But then, suddenly everything went dark. Malicious laughter was heard in a female voice that sounded like her. And then, the scene lit up, and Midnight Sparkle's attack at the Friendship Games was shown all around her.

Twilight trembled at being reminded of this horrifying incident. But she tried to remain calm, because if this was a vision of the past, she knew how it would end. Sunset Shimmer would transform and defeat Midnight.

However, Twilight was surprised and horrified when Midnight overpowered Sunset and disintegrated her.

"No..." said Twilight, backing into a wall.

"Oh yes," said Midnight Sparkle. "This time I was ready for her! Now, embrace the midnight!" She advanced slowly and menacingly on Twilight.

Twilight let out a scream of terror and wet herself with fright.

"Don't think a little pee will stop me from controlling you," Midnight said. "It will all dry off when we become one again!" She looked like she was about to enter her...

...then Twilight woke up with a jolt in her sleeping bag. "Aaaah!"

The other girls were all awake at this time.

"You all right, sugarcube?" asked Applejack. "You looked like you were havin' a bad dream."

"I was," said Twilight when she caught her breath. "About the Friendship Games."

"Oh my," said Fluttershy.

"It's all right," said Sunset. "That's in the past, and no one blames you. It was all Principal Cinch's fault."

Twilight started to feel better. Until she realized her pajama pants were wet. She felt them, and at the same time, noticed a pee smell in the air. She blushed beet red. "I wet the bed."

"It's all right, sugarcube," said Applejack. "I wet myself once when I was workin' real hard and bein' too stubborn to take a bathroom break."

"Sometimes when I get scared, I pee myself." Fluttershy blushed lightly. "It doesn't happen as much lately, but it does happen, and I hope you can feel better about it soon."

"I wet myself all the time and find it real funny," said Pinkie, who sounded cheerful but wasn't laughing at Twilight.

"Rarity?" said Twilight.

"Accidents happen, darling," said Rarity. "I promise I won't be disgusted."

"Rainbow Dash?"

"Fluttershy's accidents haven't made me like her any less," said Rainbow Dash. Fluttershy blushed and smiled.

Twilight turned toward Sunset.

"I peed in my underwear twice when I was trying to adjust to being human," said Sunset. "I also wet the bed once after I turned into a demon. But it didn't happen again because I quickly got to the point where I was only ashamed of it. The demon thing, I mean. I'm sure when you get over it, it'll stop."

Twilight giggled nervously. She wasn't sure she would get over it. But she did at least feel better about the wetting. None of her friends were showing any disgust or ridicule so she decided that, while she'd still try to avoid it of course, she'd never feel ashamed of peeing herself again.

"Now what do you say you get showered and changed?" Sunset asked.

Twilight got out of her sleeping bag, so that her wet pants were visible, and she grabbed her bag and walked into the bathroom.

...

"How is this possible? Sunset Shimmer helped me defeat you at the Friendship Games!"

"You and your friends can never truly defeat me!"

Midnight Sparkle erased all of Twilight's friends. Twilight backed into a mirror. Midnight appeared in the mirror and reached out of it.

"Midnight Sparkle's a part of you!"

Soon Twilight was floating in empty space, and a giant Midnight Sparkle hovered before her.

"I'll always be there waiting in the darkest shadows of your mind!" I'll be back, Twilight! And this time, I won't stop until I have all the magic!"

Midnight entered Twilight, who started to transform into her. "No!" said Twilight, powerless to resist the transformation.

And then she woke up on the bus. "Stop!"

"Twilight, wake up," said Spike.

"We can't stop, silly," said Pinkie. "We're not there yet!"

"Hey, are you okay?" asked Sunset. She noticed Twilight's wet shorts, but didn't say anything.

"I'm fine," said Twilight. She realized she had wet her shorts in her sleep. But, she didn't feel embarrassed about it. She had gotten past that the first time she had wet herself. She was more worried about Midnight Sparkle. Would she really take her over again?

When she met Timber Spruce at Camp Everfree, she was slightly nervous that he'd be put off by the fact that she had so obviously peed herself, and was wondering whether she should make up an excuse about spilling something on herself. But Timber didn't mention it and still really seemed to like her, so she decided it wasn't a big deal, and stayed in her wet shorts until they dried. They still smelled of pee, but she didn't care.

...

When Sunset was commenting on Twilight and Timber inside the tent, and Twilight denied it, Sunset remembered her accident on the bus, as well as her reaction when she woke up, and said "Hey, you okay? You seemed a little freaked out on the bus earlier."

"Probably had another one of her nightmares," said Spike. This was how she wet herself the first time, after all.

"I'm fine," said Twilight. "I mean, what do I have to complain about? Ever since I transferred here, everyone from CHS has been really nice and accepting, especially considering what happened at the Friendship Games."

"That wasn't your fault," said Sunset. "Principal Cinch is the one who encouraged you to try and use all that magic to win the Games for Crystal Prep. You weren't ready to have that kind of power. But it's over now. And if there's any group that's gonna forgive you for something that happened in the past, trust me, it's this one."

"You guys coming or what?" Rainbow Dash asked from the tent entrance.

"Be right there!" said Sunset. "I just want to put on some sunscreen. Huh. I could've sworn I packed it."

"Found it!" said Twilight. The sunscreen levitated in front of her. More objects in the tent were levitating. Twilight wet her shorts out of fright. It was bad enough when Midnight Sparkle was in her dreams, but was she coming out and affecting everything in the real world too?

Sunset, on the other hand, was very excited about this, and the part of her that did notice Twilight's accident thought she wet herself out of excitement rather than fear. "Did you? How is this possible?"

"I can't believe this," said Twilight. It's..."

"Amazing!" said Sunset.

"Terrible!" said Twilight at the same time.

Sunset was a little surprised, and expressed her enthusiasm while Twilight hinted at her fears of Midnight Sparkle. When Twilight was telling Sunset to keep quiet about it, Sunset sat down, realizing the reason behind Twilight's accident for the first time and feeling bad for her. "If you really don't want me to, I won't say anything."

"I really don't."

"Okay, but this isn't necessarily a bad thing!"

Twilight walked out of the tent. When she was outside, she fully noticed her wet shorts. This was the first time she had wet herself while awake. But she was less worried about this than Midnight Sparkle.

...

Twilight wasn't the only one to wet herself this trip. Rarity refused to use the outhouse or go in a bush. When it became apparent she really needed to go, Applejack told her to suck it up and either use the outhouse or go behind a bush. Rarity instead surprised everyone by peeing in her shorts. She was blushing when she finished but insisted it was cleaner, "the lesser of two evils", and that she would only do so in situations like this. And Fluttershy wet herself at Timber's campfire story.

But no one made a big deal about any of the wettings, unlike when Applejack fell into the lake. Also, Rarity was eventually persuaded to go behind a bush when the alternative was messing herself, and after this she would pee behind a push as well.

...

"I'll stay," said Twilight, following Sunset convincing her to let her help her with the whole Midnight situation. But I still don't think it's a good idea for me to be near the rest of our friends right now. Not until we know why this is happening."

"Understood," said Sunset. A rustling sound was heard. "What was that?"

An axe could be seen lifting up in the trees. Twilight and Sunset let out a scream of fright, and Twilight peed herself yet again, thinking a max axe-murderer was going after her like in a horror movie, or Gaea Everfree levitating an axe. She was relieved to see it was only Timber carrying the axe, and as they asked each other what they were doing there in the middle of the night, Timber noticed Twilight's wet shorts but didn't say anything yet. Twilight was well aware of this as well but she was used to it at this point, she was just as prone to pee accidents as Fluttershy now. She also chuckled at Timber's impression of Gloriosa.

When Timber took Twilight's hand to lead her through a shortcut back to the tents, as Sunset and Spike speculated about Timber and Gaea Everfree, Timber said "Sorry I made you... you know."

Twilight blushed lightly. "It's all right. It's been happening a lot lately, and I'm used to it."

Timber remembered the state Twilight had been in when she first arrived. "Does it only happen when you get really scared?"

"And when I have nightmares." She shuddered at the thought of Midnight Sparkle.

"Do you want a change of shorts?"

"Thanks, but I can wait till they dry, again. Besides, I'll be changing into my pajamas for bed, they can dry then."

...

When Twilight was walking through the forest, and distracted by a light, she accidentally walked into a spiderweb. She peed a little, but not much, and Sunset covered her mouth to keep her from screaming a second time.

Her shorts damp, Twilight made her way to the cave with Sunset and Spike, where they found Gloriosa to be behind the strange goings on at camp. Gloriosa tied Twilight and Sunset up with vines to keep them from interfering with her plans, and transformed into a monster. Twilight still had to go to the bathroom, but this was the last thing on her mind. She struggled to get free of the vines, straining so hard she wet herself all the way, but barely noticed before Spike bit the vines and tore them off.

After defeating Midnight Sparkle, ponying up to defeat Gloriosa dried Twilight's shorts off and removed the pee smell. After this she never had another nightmare about Midnight, nor did she wet the bed, and very rarely wet herself out of fear again.