"Simply fascinating!" Starswirl exclaimed.

"Would everypony stop trying to stab my ass!?" Chrysalis shouted indignantly.

"I'm simply being thorough, my dear."

The scalpel being held in Starswirl's magical grip levitated over to the nearby table where it dropped unceremoniously.

"It seems your carapace hardens in response to physical trauma." Starswirl stated as he scribbled some notes on a clipboard.

"Nanomachines..." Anon whispered under his breath.

An audible pop was heard as Twilight pulled her lips off Anon's cock.

"What was that, babe?" She inquired.

"Less talking, more sucking my dear Princess! I need that semen sample." Starswirl commented.

Twilight just rolled her eyes and returned to the phallus in front of her.

"AM I THE ONLY ONE HERE WHO THINKS ALL OF THIS IS INSANE!?" Chrysalis screeched.

"Quiet down, daughter dearest. The more information we can aquire the better," replied Starswirl.

"For the last time you old pervert, I'm not your DAughuh!?" Cheysalis' sentence was interrupted by her maw being forced open by Starswirl's magic.

"First of all," Starswirl began. He took note of the shape of the changeling queen's teeth. "I lack the capacity to be a pervert. My pipes have been dry since I turned 140 cycles old. And 2nd," Starswirl shut Chrysalis' maw and turned her head to observe her ears and eyes, using magic to shine a bright light to make her eyes adjust.

"We may not share blood, but since I penetrated the flower that birthed you, I'm technically your father, young lady."

Queen Chrysalis blew an errant strand of membrane that draped over her face.

"If anything that makes the nail my father."

"Well, you wouldn't say a stallion's penis is a pony's father. The father's the one driving the tool, not the tool itself," Anon interjected.

Chrysalis huffed. She was outnumbered, even if everything being said was absurd.

"Have your teeth always been flat, Chrysalis?"

The queen's eyes widened and she ran her tongue over her teeth. Tombstone shaped, with sharp canines.

"They were pointy yesterday!"

"Hmmm," Starswirl mulled. "And it's my understanding that your derriere was much less voluptuous and juicy just yesterday as well?"

"Questionable choice of adjectives aside, yes, I was much less endowed."

"And the strange compulsion to do as ordered began at that time as well, yes?"

"Do you have any idea what it all means?" Twilight asked.

"Keep sucking! Anon, for the love of the Elements, how long are you going to last?" Starswirl asked.

Anon shrugged. "Sorry, doc. I'm not trying to hold back. I kinda drained my nuts like a firehose a bit ago and I'm not particularly sensitive right now. I'll get there, though. Big Mama knows what she's doing."

Twilight smiled around Anon's dick at the compliment and redoubled her efforts, taking him even deeper in her throat.

"That reminds me; you started experiencing some changes as well, Mr. Mous, correct? Some sort of supercharged ejaculation?"

Anon nodded.

"Well, I think it's safe to say you two are having a profound effect on each other, but we still need to narrow down its intensity and longevity."

Anon felt his testes twitch. "Alright, it's coming!"

Taking her mouth off Anon's cock and pumping it with her magic, Twilight levitated over a small glass tube and held the opening towards the tip of Anon's geyser. The magic-job felt incredible on Anon's dick as it began pulsing, sending his creamy payload forward. There was enough force behind the rope that Twilight felt the tube get knocked backwards slightly. Just the first jet had managed to fill the tube and Twilight was quick enough to place her maw over the cum faucet and she began swallowing in earnest. While she sucked and swallowed she placed a cork on the tube and levitated it over to Starswirl's lab stable.

Anon for his part was having a religious experience. This orgasm wasn't powerful, but it was long. Well over a minute Anon was cumming and Twilight was swallowing, her belly distending once again as this time her stomach rather than her womb was being filled with Anon's super seed. As the flow slowed to a dribble, Twilight made sure to clean his cock with her tongue to not waste a single drop.

"Good heavens, son! I daresay that's enough splooge to impregnate half of Canterlot," Starswirl declared as he gawked at the obscene moment.

"Mmmmmm," Twilight moaned as she swallowed her final mouthful. "What the hay have you been eating, Anon? You've never tasted this good before."

A shudder ran through Anon's body as he recovered from being a human waterhose. "I don't think I've eaten anything since the bar, actually. And even before that, nothing special."

Twilight licked her lips. "Well if I didn't know any better I'd say you've been eating nothing but Hayburgers for the last week. But you can't even digest those."

"Does that not hurt?" Anon inquired as he pointed at Twilight's stomach.

"Huh?" Twilight looked down. "Not really. I mean, it feels full, but it's not painful."

As they conversed, Starswirl had been taking the sample and mixing small amounts of it with different chemicals and powders, as well as placing some on and in small machines and plates.

"Hayburgers, you say?" Using a glass stirring rod, Starswirl picked up a glob of the slime and placed it on his tongue.

Everybody else in the room lightly recoiled.

"Mmm, most peculiar. Princess Twilight, does this so-called 'hayburger' happen to contain glowberry jam?"

"Uhhh, not to my knowledge."

"Well, then perhaps it's just a coincidence because all I can taste is a childhood treat I ate almost daily; homemade glowberry jam on toast."

"Well, hayburgers are salty and savory besides the ketchup on them. Do you think it changes flavor?"

Chrysalis, having identified as chopped liver for the last ten minutes, chimed in. "If I may," she began. All the other heads in the room turned to her, suddenly remembering her presence.

"Changeling lubrication fluid can change flavor to suit the individual and circumstance. It could blow our cover if a pony notices their partner tastes too different than they normally do."

"Hmmm," Starswirl pondered. "While that certainly sounds similar, this is most certainly seminal fluid. The thaumograph detects life essence in the ejaculate, and we both tasted it from the same load. It's my understanding that only the Changeling Queen produces any kind of reproductive cells, is that not right?"

Chrysalis nodded her head. "Changeling drones only produce fluids that aid in collecting love. If they could reproduce on their own, there would be hundreds of rogue hives."

"Well, we have information, but lack the isolated variables to draw conclusions. I have a working theory, but we need to run some live tests to confirm it."

"You sayin' we need to fuck for science?" Anon asked.

Starswirl chuckled. "If you choose to put it in such a crass manor, yes. However, we'll need to isolate variables by testing multiple times."

"I think I'm understanding," Twilight stated. "Basic scientific method. We keep as many variables constant as we can while deliberately changing one so we can get accurate measurements. Although, there aren't many variables we have control over, especially when we're dealing with biology."

"Quite right, my dear. How I wish you could've been one of my students back in the day," Starswirl complimented the princess.

Twilight blushed and looked away while smiling, undoubtedly bursting inside from receiving a compliment from her childhood idol.

"We must settle for sub-optimal conditions, I'm afraid. That being said, the one variable that's likely most important is the one we have the most control; Anon's partner."

"What do you suggest?"

"Well, the changes began after mating with Chrysalis, and then again after sex with our dear Princess. So we'll want to recreate those conditions to see if more changes will come. Then we'll need a control. Somepony who has never had relations with Anon before would be preferable."

"I don't think Anon would have a problem with that."

Anon pondered the circumstances. "I mean, I'm always down to clown, but I don't think I can just pull in any random mare from a bar for this. Not exactly a walk in the park to find a mare willing to have sex with a six-foot tall monkey while being watched by a time-traveling sorcerer, the country's monarch, and a terrorist. On top of that, this lab isn't exactly the most romantic place to lay pipe."

"Can't I just be somepony else?" Inquired the changeling.

"Variables, daughter dearest," the wizard answered. "Although I sincerely doubt it will change our findings, we must be sure to account for everything we can. The equipment can be carried room to room, so we can recreate the environment. My stasis field won't even effect the thaumograph. That just leaves our final subject."

"Let's see..." Twilight began pacing the room in thought. The other individuals in attendance began to ruminate as well. "We need a mare who knows Anon, but hasn't slept with him."

"That's a pretty narrow list," Anon declared with a hint of pride.

"She has to be observant and critical to notice any changes that might occur. Somepony who's dealt with enough stress to not be scared off by such strange circumstances. And finally she has to be somepony of complete mutual trust. A mare who not only trusts one of us completely to not let them come to harm, but one we can trust to not let news of Chrysalis' return out and cause a panic."

Chrysalis and Starswirl had a minimal pool to draw on to begin with, and Anon was having trouble thinking of a mare he hadn't slept with.

Twilight gasped as recognition filled her eyes. "Ah-ha! I know just the mare!"

Author's Note

Cookie to anyone who can guess who our fourth lucky lady could be. I'll give you a hint: she's not an alicorn and she's not a main character.

Ya'll know the drill by now. Not proofread, please point out any typos or improper grammar you see. Also I plan for the next chapter to be obnoxiously long. Might split it into 2 or even 3 depending on how it goes