Twilight Sparkle is perhaps a little too overly protective of her adopted "not son," Spike. Sure, he's an adult now. At least by pony standards. Who knows how much he'll develop and change as the years go on? Worse, he's been dating Gabby lately. Twilight considers this a safety hazard for all parties involved.

But that's okay. She has a plan. She's going to study this problem, intimately, in a very hands on fashion. It's structured medical research, right?

At least if something goes wrong it will be well documented in this research journal she's keeping. But nothing is going to go wrong. She's being careful. Sort of.

Warnings: Hand written notes. Tell don't show. Accurate date logs. Measurements of semen. Lots of semen. So much semen. Anthro hands touching things that excrete semen. Notes stricken from the record. Italics.

Real trigger warnings (Spoilers!):
Incest(?). Non-con. Manipulation. Corruption. Dom/Sub. Actual rape at one point I am not kidding. NTR/cheating.

Pre-read by:
Oblivion2k (https://www.fimfiction.net/user/1113/oblivion2k)
Equimorto (https://www.fimfiction.net/user/289771/Equimorto)
Shardikku: (https://www.fimfiction.net/user/20974/Shardikku)