The Alicorn Amulet has corrupted Twilight and compelled her to take over Ponyville, leaving Trixie and Fluttershy as the only ones able to stop her.

Applejack has to prevent the last motorcycle manufacturer in the country from being ruined by its ruthless vice president and stop a plot to ruin the reputation of the biker gangs in the Sinks.

Fluttershy heads to Manehattan and ends up living with Trixie.