Author's Note

Below is the rough notes of Twilight into alicorns physiology they are unedited and unrefined. They will not be edited or proof read.

Alicorns pre-face and subject one:Luna

"These are the rough notes of Princess Twilight Sparkle, I am conducting a scientific study into the alicorns that feature in the collective works of Neokiva, a weird and eccentric unicorn mare and writer." Twilight started dictating.

"Hey! What is that supposed to mean, you make it sound like being weird and eccentric is a bad thing." Neokiva grumbled.

"Not necessarily bad, just descriptors." Twilight replied offhoofedly. "And weren't you the one who wanted to do this? Oh and thanks for funding this by the way." Twilight added.

"Yes, I did. So I had a unified theory of alicorns from my worlds so people can use this as a guide. you are also welcome." Neokiva agreed.

"Do we have to do this?" Luna griped

"I don't see why your complaining Luna, a lot of the studies are hooves on and fun." Neokiva continued

"We, for one art most concerned and self conscious, what will ponies say when they discover some of our secrets. What will 'Tia say." Luna asked.

"Princess Celestia was most enthusiastic and very receptive to it and about the deal I did to secure her participation I wonder why?" Neokiva interjected confusedly.

"What did you promise her?" Twilight asked eyeing Neokiva warily.

"I'll tell you later if I am able, oh and if you don't hear from me in five days come to canterlot castle and find me as I may need assistance and bring plenty of water as well as an ice pack I'm certain I'll need it." Neokiva trotted out. A few minutes later she picked up one of the bottles of lube, before rushing out again.

"Okay~ then let's get continue!" Twilight exclaimed.

"Along the way I shall interview subjects and produce evidence where possible or cite another source, peer reviewed by myself." Twilight dxplained

Today our subject is Princess Luna.

"Twilight art thou sure that we need to do this?" Luna grimaced as Twilight attached electrodes to her body.

"Yes we do for science!!!" Twilight gleefully exclaimed while Luna grumbled something under her breath.

"What was that dear?" Twilight asked sweetly with a smile on her face.

"Nothing sweety! I'm just so excited to help you with your experiment." Luna laughed nervously.

"Good, let's get started." Twilight said as she levitated a tape measure and a large plain dildo. Twilight then liberally slathered it in lubricant. Luna also found her levitated up to a ring with four hoof cuffs and locked Luna's legs in each. Twilight slipped a magic nullifying ring onto Luna's horn. The dildo floated behind Luna's body and sight, Luna felt a tugging on her tail as a foreign object prodded against Luna's backdoor and only had a second to brace as the offending object shot up in Twilight's magic. Luna grunted from the sudden intrusion, Twilight moved the dildo in angles subject Luna closed her eyes moaning ever so slightly.

Luna turned to Twilight and levelled a glare at her, "Having fun are you dear?" Luna asked Twilight.

"Of course!" Twilight replied cheerfully. Luna felt a pleasant pressure in her abused anus as the dildo moved and caressed the walls of her rectum. Luna felt a wave of pleasure going through her body a shiver up her spine as her cock made it's appearance soon after becoming turgidly erect.

"Subject Luna apparently has a penis whether or not it can impregnate mares has yet to be determined. Also to be determined is if she is a unique case among Alicorns."

"It's almost as if I'm a futanari or something." Luna commented dryly.

Twilight dived underneath Luna's testicals and looked up and sure enough she spotted Luna's drenched marehood winking. Twilight dragged her tongue along it sampling Luna's taste.

"Subject Luna is correct she is a futanari and she appears to be in heat."

"Mmmnn you taste like blueberries." Twilight moaned and licked her lips as Luna shuddered at the feeling of Twilight's tongue against her marehood. Twilight measured Luna's length with the tape.

"Oh my three feet of meat!" Twilight squealed with excitement.

"Now to prepare for a long term experiment." Twilight giggled as she spun around and lifted her tail revealing her own soaking wet winking marehood, causing Luna to strain at her restraints " Subject is reacting similar to how a stallion would, when a mare presents themselves during heat. " Twilight used her magic to part her lower lips backing up she took Luna's cock in her magic and guides the tip to her soaked nethers sliding it in as she backed into Luna who was trying to buck her hips forward to feel the rest of Twilight's vagina.

Giggling Twilight hilted on Luna's cock as she did Twilight gasped, feeling the tip flaring as it was prodding against her cirvix. Luna was gasping as her sensitive member rubbed along her mare's tight tunnel. Luna felt the warmth sliding up her cock gasping as good air hit it. Before she felt it re-enveloped
In Twilight's warmth, causing her to moan loudly and more frequently. Twilight rhythmically pumped up and down on Luna's shaft adding her own grunting and moaning to the o D's filling the room. Twilight pressed her back into Luna's barrel and leant her head back, Luna took advantage of her new position to lick Twilight's horn curling her long tongue around the spirals eliciting squeaks and moans from her, as Luna thrust into her tight pussy.

Twilight and Luna where both in Elysium as Luna took Twilight's horn into her mouth waves of pleasure crashed over their bodies as Luna felt a pressure building in her balls, "T-twilight I am almost there don't stop!" Twilight redoubled her efforts thrusting down harder as she tightened her marehood around Luna's rod, the two mare's moaning reaching a crescendo. Luna hilted as Twilight's marehood tightened her flared stallion hood it breached Twilight's cirvix cum rushed up her cock and pulsed as ropes of cum filled Twilight's womb with Luna's seed again and again with each pulse both mares screaming in ecstacy as they came.

"Experiment prep concluded, expected result, our own little foal." Twilight released Luna's restraints and the lunar princess finally wrapped her forehooves around Twilight as Twilight nuzzled her.