Opaline has now discovered that the Mane 5 can use the Unity Crystals to travel to other lands when they are in their time of need and sends Misty on a mission to make sure they never return. But Misty cannot betray her new friends... especially when they have a new mission to go on.

This time, they (for the first time) meet Mystery Incorporated, a team of mystery solvers composing of Scooby-Doo, Shaggy, Fred, Velma and Daphne, and help them solve the most frighteningly-funny mystery of the gang's careers.

The Scream Team, plus their new pony friends, are headed to a haunted bayou island to investigate the ghost of Moonscar the pirate. But it turns out the swashbuckler's spirit isn't the only creepy character on the island. The sleuths also meet up with cat creatures and zombies... and it looks like, for the first time, these ghouls might actually be real.

Just as things start to get really spooky, Misty along with Shaggy and Scooby, save the day with help from a surprisingly spooky source. With wholesome characters and brand-new friendships forming, the second installment of G5 Adventures dishes up equal portions of music, laughs, thrills and chills.

(S1E2 of G5 Adventures, and part one of two entertwined stories, intertwined with one another)