Adaptation Crossover of various Mickey Mouse and Friends media , Classic Shorts, Mickey Mouse Clubhouse, Mickey Mouse (2013) etc

Main Cast:
Twilight Sparkle as Mickey Mouse
Fluttershy as Minnie Mouse
Spike as Donald Duck
Rarity as Daisy Duck
Pinkie Pie as Goofy
Gummy as Pluto

Minor Cast:
Discord as Pete
Diamond Tiara and Silver Spoon as Chip and Dale
Dr Whooves as Professor Von Drake
Rainbow Dash as Mortimer
CMCs as Donald's Nephews
Ember as Scrooge Mcduck
Cheese Sandwhich as Clarabelle Cow
Silverstream, Sandbar and Smolder (Snorkler, Miner, Practical) as The Three Little Pigs (Fifer, Fiddler and Practical)
Gallus as The Big Bad Wolf

VERY Minor Cast (Obscure Characters)
Tank as Spike The Bee (One of Donald Duck's enemies)
Button Mash And Rumble as Morty and Ferdie

(Some characters like Pluto, Goofy, Chip and Dale are based on personality while characters like Donald, Daisy, Mickey, Minnie and Mortimer are based on ships)